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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
36
pubmed:dateCreated
2007-10-26
pubmed:abstractText
Here, we report biomodification of temperature-responsive culture surfaces with biotinylated biomolecules utilizing streptavidin and biotinylation of the surfaces. Poly(N-isopropylacrylamide-co-2-carboxyisopropylacrylamide) was covalently grafted onto tissue culture polystyrene (TCPS) dishes. Biotinylated Arg-Gly-Asp-Ser (RGDS) peptides with different spacer lengths (biotin-conjugated G(n)RGDS (n=1,6,12,16)) were examined. Human umbilical vein endothelial cells (HUVECs) adhered and were well spread on G(12)RGDS-immobilized surfaces in the absence of serum at 37 degrees C, while much less cell adhesion was observed with the other peptides. Adhered HUVECs were detached on reducing temperature to 20 degrees C, or on adding free RGDS peptide. Interestingly, cell detachment was accelerated by applying both these techniques. Consequently, by optimizing the spacer length, biomolecules can be functionally immobilized onto thermoresponsive surfaces via the affinity binding between avidin and biotin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0142-9612
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5471-6
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
The use of biotin-avidin binding to facilitate biomodification of thermoresponsive culture surfaces.
pubmed:affiliation
Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't