Linked Life Data

17855651

Source:http://linkedlifedata.com/resource/pubmed/id/17855651

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2007-11-20
pubmed:abstractText
Expression of Urate transporter 1 (URAT1/SLC22A12) is restricted to the proximal tubules in the kidney, where it is responsible for the tubular reabsorption of urate. To elucidate the mechanism underlying its tissue-specific expression, the transcriptional regulation of the hURAT1 and mUrat1 genes was investigated. Hepatocyte nuclear factor 1 alpha (HNF1alpha) and HNF1beta positively regulate minimal promoter activity of the URAT1 gene as shown by reporter gene assays. Electrophoretic mobility shift assays revealed binding of HNF1alpha and/or HNF1beta to the HNF1 motif in the hURAT1 promoter. Furthermore, the mRNA expression of Urat1 is reduced in the kidneys of Hnf1alpha-null mice compared with wild-type mice, confirming the indispensable role of HNF1alpha in the constitutive expression of URAT1 genes. It was also shown that the proximal promoter region of mUrat1 was hypermethylated in the liver and kidney medulla, whereas this region was relatively hypomethylated in the kidney cortex. These methylation profiles are in a good agreement with the proximal tubule-restricted expression of mUrat1 in the kidney cortex. Taken together, these results strongly suggest that tissue-specific expression of the URAT1 genes is coordinately regulated by the transcriptional activation by HNF1alpha/HNF1beta heterodimer and repression by DNA methylation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1521-0111
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1619-25
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:17855651-Animals, pubmed-meshheading:17855651-Base Sequence, pubmed-meshheading:17855651-Cell Line, pubmed-meshheading:17855651-DNA Methylation, pubmed-meshheading:17855651-Female, pubmed-meshheading:17855651-Gene Expression Regulation, pubmed-meshheading:17855651-Hepatocyte Nuclear Factor 1-alpha, pubmed-meshheading:17855651-Hepatocyte Nuclear Factor 1-beta, pubmed-meshheading:17855651-Humans, pubmed-meshheading:17855651-Male, pubmed-meshheading:17855651-Mice, pubmed-meshheading:17855651-Mice, Knockout, pubmed-meshheading:17855651-Molecular Sequence Data, pubmed-meshheading:17855651-Organic Anion Transporters, pubmed-meshheading:17855651-Organic Cation Transport Proteins, pubmed-meshheading:17855651-Tissue Distribution, pubmed-meshheading:17855651-Transcriptional Activation
pubmed:year
2007
pubmed:articleTitle
Regulation of tissue-specific expression of the human and mouse urate transporter 1 gene by hepatocyte nuclear factor 1 alpha/beta and DNA methylation.
pubmed:affiliation
Department of Molecular Pharmacokinetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
pubmed:publicationType
Journal Article, Comparative Study