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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2007-10-1
pubmed:abstractText
Brain derived neurotrophic factor (BDNF) increases the levels of pre-pro-thyrotropin releasing hormone (TRH) mRNA in fetal rodent hypothalamic neurons that express TrkB receptors. The present studies aimed at better understanding the role of BDNF in establishing and maintaining the TRH phenotype in hypothalamic neurons during early development. To determine where BDNF regulates the expression of pre-pro-TRH mRNA in vivo, we compared the hypothalamic distribution of pre-pro-TRH mRNA to that of TrkB mRNA. Full-length TrkB (FL-TrkB) mRNA was detected earlier in development than pre-pro-TRH mRNA in the region that gives rise to the paraventricular nucleus of the hypothalamus (PVN). We also evaluated the effects of BDNF on the expression of pre-pro-TRH mRNA in vitro. BDNF up-regulated the levels of pre-pro-TRH mRNA in primary cell cultures obtained from the hypothalamus or the PVN of 17 days old fetuses or newborn rats. This effect was abolished by PD98059, an inhibitor of the mitogen-activated protein kinase kinase (MEK) 1/2 or 5. The effect of BDNF on pre-pro-TRH mRNA levels was reversible. The continuous application of BDNF led to a desensitization of the response at day 10 in vitro, an effect that correlated with a drop in the levels of FL-TrkB protein. In conclusion, BDNF enhances the expression of pre-pro-TRH mRNA in PVN neurons. This effect is reversible, decreases with time, and requires an active MEK. BDNF may contribute to the enhancement of pre-pro-TRH mRNA expression in the hypothalamic PVN during development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
1174
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
28-38
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:17854778-Animals, pubmed-meshheading:17854778-Animals, Newborn, pubmed-meshheading:17854778-Brain-Derived Neurotrophic Factor, pubmed-meshheading:17854778-Carcinoma, Medullary, pubmed-meshheading:17854778-Female, pubmed-meshheading:17854778-Gene Expression Regulation, Developmental, pubmed-meshheading:17854778-Hypothalamus, pubmed-meshheading:17854778-Male, pubmed-meshheading:17854778-Neurons, pubmed-meshheading:17854778-Paraventricular Hypothalamic Nucleus, pubmed-meshheading:17854778-Pregnancy, pubmed-meshheading:17854778-Protein Precursors, pubmed-meshheading:17854778-RNA, Messenger, pubmed-meshheading:17854778-Rats, pubmed-meshheading:17854778-Rats, Wistar, pubmed-meshheading:17854778-Receptor, trkB, pubmed-meshheading:17854778-Signal Transduction, pubmed-meshheading:17854778-Thyroid Neoplasms, pubmed-meshheading:17854778-Thyrotropin-Releasing Hormone, pubmed-meshheading:17854778-Tumor Cells, Cultured
pubmed:year
2007
pubmed:articleTitle
BDNF up-regulates pre-pro-TRH mRNA expression in the fetal/neonatal paraventricular nucleus of the hypothalamus. Properties of the transduction pathway.
pubmed:affiliation
Centro de Ingeniería Genética y Biotecnología, La Habana, Cuba.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't