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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2007-11-1
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pubmed:abstractText |
Therapeutic angiogenesis is one of the major approaches in designing new therapies for cardiovascular diseases. vpVEGF was purified from Vipera palestinae venom using two steps of reverse-phase HPLC. Structurally, vpVEGF belongs to the VEGF-F1 family of snake venom proteins, and potently stimulated dHMVEC proliferation in a VEGFR-2 dependent manner. This growth factor appeared to be a chemoattractant for migration of these cells and stimulated their radial migration in a collagen gel. The stimulatory effect on dHMVEC was correlated with activation of the MAPK Erk1/2 signaling pathway. In vivo vpVEGF induced angiogenesis in a Japanese quail assay and in a Matrigel plug assay in mice. Although in the quail assay vpVEGF showed lower activity than hrVEGF-A165 in mammalian-related systems there were no significant differences. The experiments with dHMVEC, as well as angiogenesis in vivo suggest that the pro-angiogenic effect of vpVEGF is related to its interaction with VEGFR-2 (flk-1).
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0897-7194
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
108-17
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:17852405-Amino Acid Sequence,
pubmed-meshheading:17852405-Animals,
pubmed-meshheading:17852405-Cell Movement,
pubmed-meshheading:17852405-Cell Proliferation,
pubmed-meshheading:17852405-Chemotaxis,
pubmed-meshheading:17852405-Collagen,
pubmed-meshheading:17852405-Coturnix,
pubmed-meshheading:17852405-Drug Combinations,
pubmed-meshheading:17852405-Endothelial Cells,
pubmed-meshheading:17852405-Female,
pubmed-meshheading:17852405-Laminin,
pubmed-meshheading:17852405-MAP Kinase Signaling System,
pubmed-meshheading:17852405-Mice,
pubmed-meshheading:17852405-Mice, Inbred C57BL,
pubmed-meshheading:17852405-Molecular Sequence Data,
pubmed-meshheading:17852405-Neovascularization, Pathologic,
pubmed-meshheading:17852405-Proteoglycans,
pubmed-meshheading:17852405-Vascular Endothelial Growth Factor A,
pubmed-meshheading:17852405-Vascular Endothelial Growth Factor Receptor-2,
pubmed-meshheading:17852405-Venoms,
pubmed-meshheading:17852405-Viperidae
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pubmed:year |
2007
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pubmed:articleTitle |
VEGF-related protein isolated from Vipera palestinae venom, promotes angiogenesis.
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pubmed:affiliation |
Department of Neuroscience, Center for Neurovirology, School of Medicine, Temple University, 1900 N. 12th Street, Philadelphia, PA 19122, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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