Source:http://linkedlifedata.com/resource/pubmed/id/17851547
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0004561,
umls-concept:C0017262,
umls-concept:C0020205,
umls-concept:C0021027,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0086022,
umls-concept:C0086418,
umls-concept:C0086860,
umls-concept:C0185117,
umls-concept:C0205314,
umls-concept:C0442335,
umls-concept:C0679622,
umls-concept:C1705099,
umls-concept:C2911684
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| pubmed:issue |
23
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| pubmed:dateCreated |
2007-11-20
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| pubmed:abstractText |
The expression of genes specifically in B cells is of great interest in both experimental immunology as well as in future clinical gene therapy. We have constructed a novel enhanced B cell-specific promoter (Igk-E) consisting of an immunoglobulin kappa (Igk) minimal promoter combined with an intronic enhancer sequence and a 3' enhancer sequence from Ig genes. The Igk-E promoter was cloned into a lentiviral vector and used to control expression of enhanced green fluorescent protein (eGFP). Transduction of murine B-cell lymphoma cell lines and activated primary splenic B cells, with IgK-E-eGFP lentivirus, resulted in expression of eGFP, as analysed by flow cytometry, whereas expression in non-B cells was absent. The specificity of the promoter was further examined by transducing Lin(-) bone marrow with Igk-E-eGFP lentivirus and reconstituting lethally irradiated mice. After 16 weeks flow cytometry of lymphoid tissues revealed eGFP expression by CD19+ cells, but not by CD3+, CD11b+, CD11c+ or Gr-1+ cells. CD19+ cells were comprised of both marginal zone B cells and recirculating follicular B cells. Activated human peripheral mononuclear cells were also transduced with Igk-E-eGFP lentivirus under conditions of selective B-cell activation. The Igk-E promoter was able to drive expression of eGFP only in CD19+ cells, while eGFP was expressed by both spleen focus-forming virus and cytomegalovirus constitutive promoters in CD19+ and CD3+ lymphocytes. These data demonstrate that in these conditions the Igk-E promoter is cell specific and controls efficient expression of a reporter protein in mouse and human B cells in the context of a lentiviral vector.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
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| pubmed:issn |
0969-7128
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
14
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1623-31
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:17851547-Animals,
pubmed-meshheading:17851547-B-Lymphocytes,
pubmed-meshheading:17851547-Cell Line,
pubmed-meshheading:17851547-Female,
pubmed-meshheading:17851547-Flow Cytometry,
pubmed-meshheading:17851547-Gene Expression,
pubmed-meshheading:17851547-Gene Therapy,
pubmed-meshheading:17851547-Genetic Engineering,
pubmed-meshheading:17851547-Genetic Vectors,
pubmed-meshheading:17851547-Green Fluorescent Proteins,
pubmed-meshheading:17851547-HIV-1,
pubmed-meshheading:17851547-Humans,
pubmed-meshheading:17851547-Immunoglobulins,
pubmed-meshheading:17851547-Male,
pubmed-meshheading:17851547-Mice,
pubmed-meshheading:17851547-Mice, Inbred C57BL,
pubmed-meshheading:17851547-Promoter Regions, Genetic,
pubmed-meshheading:17851547-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17851547-Transduction, Genetic,
pubmed-meshheading:17851547-Transgenes
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| pubmed:year |
2007
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| pubmed:articleTitle |
Cell-specific and efficient expression in mouse and human B cells by a novel hybrid immunoglobulin promoter in a lentiviral vector.
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| pubmed:affiliation |
Molecular Immunology Unit, Wolfson Centre for Gene Therapy of Childhood Disease, UCL Institute of Child Health, London, UK.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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