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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2007-9-13
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pubmed:abstractText |
The highly diversified variable regions of immunoglobulin (Ig) molecules contain immunogenic determinants denoted idiotopes. We have previously reported that T cells from multiple sclerosis (MS) patients recognize IgG from autologous cerebrospinal fluid (CSF), and mapped a T-cell epitope to an IgG idiotope. To test the ability of CSF IgG molecules to elicit a broad polyclonal T-cell response in MS, we have analysed T-cell responses in the blood and CSF against idiotope peptides spanning complementarity determining region (CDR) 3 and somatic mutations within the variable regions of monoclonal CSF IgG. Consistent with a diversified idiotope-specific T-cell repertoire, CD4(+) T cells from both patients recognized several idiotope peptides presented by HLA-DR molecules. Mutations were critical for T-cell recognition, as T cells specific for a mutated CDR1 peptide did not recognize corresponding germline-encoded peptides. One T-cell clone recognized both an idiotope peptide and the B-cell clone expressing this idiotope, compatible with endogenous processing and presentation of this idiotope by B cells. These results suggest that mutated CSF IgG from MS patients carry several T-cell epitopes, which could mediate intrathecal IgG production and inflammation in MS through idiotope-driven T-B-cell collaboration.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Complementarity Determining Regions,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/HLA Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DRB1 Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Idiotypes,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0300-9475
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
393-401
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:17850583-Adult,
pubmed-meshheading:17850583-CD4-Positive T-Lymphocytes,
pubmed-meshheading:17850583-Cell Proliferation,
pubmed-meshheading:17850583-Complementarity Determining Regions,
pubmed-meshheading:17850583-Epitopes, T-Lymphocyte,
pubmed-meshheading:17850583-Female,
pubmed-meshheading:17850583-HLA Antigens,
pubmed-meshheading:17850583-HLA-DR Antigens,
pubmed-meshheading:17850583-HLA-DRB1 Chains,
pubmed-meshheading:17850583-Humans,
pubmed-meshheading:17850583-Immunoglobulin G,
pubmed-meshheading:17850583-Immunoglobulin Idiotypes,
pubmed-meshheading:17850583-Immunoglobulin Variable Region,
pubmed-meshheading:17850583-Interferon-gamma,
pubmed-meshheading:17850583-Interleukins,
pubmed-meshheading:17850583-Male,
pubmed-meshheading:17850583-Multiple Sclerosis,
pubmed-meshheading:17850583-Peptide Fragments,
pubmed-meshheading:17850583-Tumor Necrosis Factor-alpha
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pubmed:year |
2007
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pubmed:articleTitle |
T cells from multiple sclerosis patients recognize multiple epitopes on Self-IgG.
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pubmed:affiliation |
Institute of Immunology, Rikshospitalet-Radiumhospitalet Medical Center, University of Oslo, Oslo, Norway.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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