Linked Life Data

17844999

Source:http://linkedlifedata.com/resource/pubmed/id/17844999

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2007-10-1
pubmed:abstractText
In the genome of Bacillus amyloliquefaciens FZB42, three operons pks1, pks2, and pks3 were identified which encode the biosynthesis of polyketides. pks1 and pks3 have been attributed to the production of bacillaene and difficidin/oxydifficidin, respectively, while the pks2 product remained hitherto unknown. Mass spectrometric analysis of the culture filtrates of the wild-type B. amyloliquefaciens FZB42 and mutants revealed pks2-specific metabolites. By combination of the mass spectrometric and UV/vis data with a database search, these compounds were attributed to four members of the macrolactin family, macrolactin A and D as well as 7-O-malonyl- and 7-O-succinyl-macrolactin. This conclusion was verified by the isolation and structure elucidation of macrolactin A using mass spectrometric and 2D-NMR studies. Macrolactin biosynthesis was investigated using feeding experiments with (13)C-acetate. (13)C-labelled macrolactin A revealed an alternating labelling of its carbon skeleton with (13)C, indicating that acetate/malonate was used as the sole precursor. The macrolactin structure is compatible with the domain organization of the pks2-operon. Similarly to pks1 and pks3, pks2 is a modular polyketide synthase system of type I which exhibits a trans-acyltransferase architecture using a discrete acyltransferase enzyme iteratively in the assembly of macrolactin. Finally, the potential for macrolactin production on a genetic and metabolic basis was found to be widely distributed among Bacillus amyloliquefaciens strains.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0163-3864
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1417-23
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Macrolactin is the polyketide biosynthesis product of the pks2 cluster of Bacillus amyloliquefaciens FZB42.
pubmed:affiliation
Institut für Chemie, Technishe Universität Berlin, Berlin, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't