Source:http://linkedlifedata.com/resource/pubmed/id/17826339
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2007-10-23
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| pubmed:abstractText |
The influence of human leukocyte antigens (HLA) on the immune response is well established. We investigated the regulatory role of HLA-DRB1 alleles on cytokine response to live M. tuberculosis and its culture filtrate antigen (CFA) in normal healthy subjects (NHS) and pulmonary tuberculosis (PTB) patients. Th1 (IFN-gamma and IL-12p40), Th2 (IL-4 and IL-5), pro-inflammatory (IL-6 and IL-8) and anti-inflammatory (TGF-beta and IL-10) cytokines were measured by ELISA in 72-h-old peripheral blood mononuclear cell culture supernatants from 58 NHS and 48 PTB patients. HLA-DRB1 genotyping was carried out by polymerase chain reaction and dot-blot hybridization with biotinylated sequence-specific oligonucleotide probes and detection by chemiluminescence. In response to live M. tuberculosis and CFA, significantly increased levels of IL-6, IL-8 and TGF-beta and decreased IFN-gamma, IL-12p40 and IL-10 were seen in PTB patients compared to NHS. We observed a significantly increased IFN-gamma response in HLA-DRB1*03-positive NHS (p=0.03) and decreased IFN-gamma response in HLA-DRB1*15-positive patients (p=0.04) than respective allele-negative individuals. An increased level of IL-12p40 in DRB1*10 (p=0.02) and IL-10 in DRB1*12- (p=0.03) positive NHS and an increased level of IL-6 in DRB1*04- (p=0.02) positive patients were observed. The study suggests that HLA-DRB1 alleles differentially modulate the various cytokine responses to M. tuberculosis antigens, which may influence the cellular and humoral immune responses to M. tuberculosis infection in a susceptible host.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DRB1 Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Inflammation Mediators,
http://linkedlifedata.com/resource/pubmed/chemical/Mycobacterium tuberculosis antigens
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
1472-9792
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
87
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
544-50
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:17826339-Adult,
pubmed-meshheading:17826339-Antigens, Bacterial,
pubmed-meshheading:17826339-Cells, Cultured,
pubmed-meshheading:17826339-Cytokines,
pubmed-meshheading:17826339-Female,
pubmed-meshheading:17826339-Gene Frequency,
pubmed-meshheading:17826339-Genotype,
pubmed-meshheading:17826339-HLA-DR Antigens,
pubmed-meshheading:17826339-HLA-DRB1 Chains,
pubmed-meshheading:17826339-Humans,
pubmed-meshheading:17826339-Immunity, Cellular,
pubmed-meshheading:17826339-Inflammation Mediators,
pubmed-meshheading:17826339-Male,
pubmed-meshheading:17826339-Middle Aged,
pubmed-meshheading:17826339-Mycobacterium tuberculosis,
pubmed-meshheading:17826339-Th1 Cells,
pubmed-meshheading:17826339-Th2 Cells,
pubmed-meshheading:17826339-Tuberculosis, Pulmonary
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| pubmed:year |
2007
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| pubmed:articleTitle |
Influence of HLA-DRB1 alleles on Th1 and Th2 cytokine response to Mycobacterium tuberculosis antigens in pulmonary tuberculosis.
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| pubmed:affiliation |
Tuberculosis Research Centre, Indian Council of Medical Research, Mayor V.R. Ramanathan Road, Chetput, Chennai 600031, India. psraj21@hotmail.com
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| pubmed:publicationType |
Journal Article
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