Source:http://linkedlifedata.com/resource/pubmed/id/17825790
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2007-9-26
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pubmed:abstractText |
Bcl-2 plays a pivotal role in the control of cell death and is down-regulated in trichosanthin (TCS)-induced cell apoptosis. Because Bcl-2 expression is regulated by the transcription factor cyclic AMP response element-binding protein (CREB), we investigated the role of CREB activation in TCS-induced Hela cells apoptosis. Our results showed that TCS-caused Hela cell apoptosis was accompanied by the decrease of Bcl-2 and phosphorylated CREB protein levels. Interesting, this inhibitive effect can be abolished by the combined treatment of TCS/cAMP agonists. Furthermore, TCS-mediated Bcl-2 protein was abrogated by the suppression of CREB expression with antisense treatment, and blocking the interaction between CREB-binding protein and the Bcl-2 cyclic AMP-responsive element (CRE) by a CRE decoy oligonucleotide. Therefore, these data support the hypothesis that CREB plays a critical role in the regulation of Bcl-2 expression in TCS-induced Hela cell death.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents, Phytogenic,
http://linkedlifedata.com/resource/pubmed/chemical/CREB-Binding Protein,
http://linkedlifedata.com/resource/pubmed/chemical/CREBBP protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/Trichosanthin
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-291X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
363
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
101-5
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pubmed:meshHeading |
pubmed-meshheading:17825790-Antineoplastic Agents, Phytogenic,
pubmed-meshheading:17825790-Apoptosis,
pubmed-meshheading:17825790-CREB-Binding Protein,
pubmed-meshheading:17825790-Dose-Response Relationship, Drug,
pubmed-meshheading:17825790-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:17825790-HeLa Cells,
pubmed-meshheading:17825790-Humans,
pubmed-meshheading:17825790-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:17825790-Trichosanthin
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pubmed:year |
2007
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pubmed:articleTitle |
CREB-mediated Bcl-2 expression in trichosanthin-induced Hela cell apoptosis.
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pubmed:affiliation |
Institute of Cell Biology, Zhejiang University, Hangzhou 310058, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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