Linked Life Data

17804756

Source:http://linkedlifedata.com/resource/pubmed/id/17804756

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2007-9-6
pubmed:abstractText
The phytochemical resveratrol contained in red grapes has been shown to inhibit prostate cancer cell growth, in part, through its antioxidant activity. Muscadine grapes contain unique phytochemical constituents compared with other grapes and are potentially a source for novel compounds with antitumor activities. We compared the antitumor activities of muscadine grape skin extract (MSKE), which we show contains no resveratrol, with that of resveratrol using primary cultures of normal prostate epithelial cells (PrEC) and the prostate cancer cell lines RWPE-1, WPE1-NA22, WPE1-NB14, and WPE1-NB26, representing different stages of prostate cancer progression. MSKE significantly inhibited tumor cell growth in all transformed prostate cancer cell lines but not PrEC cells. Prostate tumor cell lines, but not PrEC cells, exhibited high rates of apoptosis in response to MSKE through targeting of the phosphatidylinositol 3-kinase-Akt and mitogen-activated protein kinase survival pathways. The reduction in Akt activity by MSKE is mediated through a reduction in Akt transcription, enhanced proteosome degradation of Akt, and altered levels of DJ-1, a known regulator of PTEN. In contrast to MSKE, resveratrol did not induce apoptosis in this model but arrested cells at the G(1)-S phase transition of the cell cycle associated with increased expression of p21 and decreased expression of cyclin D1 and cyclin-dependent kinase 4 proteins. These results show that MSKE and resveratrol target distinct pathways to inhibit prostate cancer cell growth in this system and that the unique properties of MSKE suggest that it may be an important source for further development of chemopreventive or therapeutic agents against prostate cancer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8396-405
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:17804756-Antineoplastic Agents, Phytogenic, pubmed-meshheading:17804756-Apoptosis, pubmed-meshheading:17804756-Cell Aging, pubmed-meshheading:17804756-Cell Proliferation, pubmed-meshheading:17804756-Gene Expression Regulation, Neoplastic, pubmed-meshheading:17804756-Humans, pubmed-meshheading:17804756-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:17804756-Male, pubmed-meshheading:17804756-Oncogene Protein v-akt, pubmed-meshheading:17804756-Oncogene Proteins, pubmed-meshheading:17804756-Plant Extracts, pubmed-meshheading:17804756-Prostatic Neoplasms, pubmed-meshheading:17804756-Protein Processing, Post-Translational, pubmed-meshheading:17804756-Seeds, pubmed-meshheading:17804756-Signal Transduction, pubmed-meshheading:17804756-Stilbenes, pubmed-meshheading:17804756-Tumor Cells, Cultured, pubmed-meshheading:17804756-Vitis
pubmed:year
2007
pubmed:articleTitle
Inhibition of prostate cancer growth by muscadine grape skin extract and resveratrol through distinct mechanisms.
pubmed:affiliation
Laboratory of Cellular Regulation and Carcinogenesis, National Cancer Institute, NIH, Bethesda, MD 20892, USA.
pubmed:publicationType
Journal Article, Comparative Study, Evaluation Studies, Research Support, N.I.H., Intramural