Linked Life Data

17804728

Source:http://linkedlifedata.com/resource/pubmed/id/17804728

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2007-9-6
pubmed:abstractText
Magnetic nanoparticles (MNP) with a diameter of 8 nm were modified with different generations of polyamidoamine (PAMAM) dendrimers and mixed with antisense survivin oligodeoxynucleotide (asODN). The MNP then formed asODN-dendrimer-MNP composites, which we incubated with human tumor cell lines such as human breast cancer MCF-7, MDA-MB-435, and liver cancer HepG2 and then analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, quantitative reverse transcription-PCR, Western blotting, laser confocal microscopy, and high-resolution transmission electron microscopy. Results showed that the asODN-dendrimer-MNP composites were successfully synthesized, can enter into tumor cells within 15 min, caused marked down-regulation of the survivin gene and protein, and inhibited cell growth in dose- and time-dependent means. No.5 generation of asODN-dendrimer-MNP composites exhibits the highest efficiency for cellular transfection and inhibition. These results show that PAMAM dendrimer-modified MNPs may be a good gene delivery system and have potential applications in cancer therapy and molecular imaging diagnosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8156-63
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:17804728-Cell Proliferation, pubmed-meshheading:17804728-Cell Survival, pubmed-meshheading:17804728-Dendrimers, pubmed-meshheading:17804728-Gene Therapy, pubmed-meshheading:17804728-Gene Transfer Techniques, pubmed-meshheading:17804728-HL-60 Cells, pubmed-meshheading:17804728-Humans, pubmed-meshheading:17804728-Inhibitor of Apoptosis Proteins, pubmed-meshheading:17804728-Magnetics, pubmed-meshheading:17804728-Metal Nanoparticles, pubmed-meshheading:17804728-Microtubule-Associated Proteins, pubmed-meshheading:17804728-Models, Biological, pubmed-meshheading:17804728-Neoplasm Proteins, pubmed-meshheading:17804728-Neoplasms, pubmed-meshheading:17804728-Oligodeoxyribonucleotides, Antisense, pubmed-meshheading:17804728-Treatment Outcome, pubmed-meshheading:17804728-Tumor Cells, Cultured
pubmed:year
2007
pubmed:articleTitle
Dendrimer-modified magnetic nanoparticles enhance efficiency of gene delivery system.
pubmed:affiliation
Department of Bio-Nano-Science and Engineering, National Key Laboratory of Nano/Micro Fabrication Technology, Shanghai Jiao Tong University, Shanghai, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies