Source:http://linkedlifedata.com/resource/pubmed/id/17804253
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2007-11-9
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| pubmed:abstractText |
A transgenic cell line for the detection of salmon interferons (IFNs) has been established. It is based on a CHSE-214 cell line containing a reporter construct expressing firefly luciferase under the control of the rainbow trout promoter for the IFN-induced Mx1 gene. This cell line, named CHSE-Mx10, showed IFN-induced luciferase expression after more than 80 passages, confirming the stability of this cell line. Interestingly, the Mx promoter was shown to respond to both salmon IFN-alpha/beta and trout IFN-gamma in a dose-dependent manner, while there was no response to TNF-alpha and IL-1beta. IFN-alpha/beta activity could be measured at a range of 9-150 U/ml, and IFN-gamma showed activity between 10 and 100 ng/ml. The reproducibility of both responses was good. The CHSE-Mx10 reporter system constitutes a versatile tool to study the induction and regulation of IFN signaling in teleost fish. A preliminary study presented herein suggests that both infectious pancreas necrosis virus (IPNV) and salmon pancreas disease virus (SPDV) may block activation of the Mx promoter in CHSE-Mx10 stimulated with IFN-alpha/beta.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Type I,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases, Firefly,
http://linkedlifedata.com/resource/pubmed/chemical/myxovirus resistance proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
1050-4648
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
23
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1294-303
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:17804253-Animals,
pubmed-meshheading:17804253-Birnaviridae Infections,
pubmed-meshheading:17804253-Cell Line,
pubmed-meshheading:17804253-Cytokines,
pubmed-meshheading:17804253-Fish Diseases,
pubmed-meshheading:17804253-GTP-Binding Proteins,
pubmed-meshheading:17804253-Gene Expression Profiling,
pubmed-meshheading:17804253-Gene Expression Regulation,
pubmed-meshheading:17804253-Gene Transfer Techniques,
pubmed-meshheading:17804253-Infectious pancreatic necrosis virus,
pubmed-meshheading:17804253-Interferon Type I,
pubmed-meshheading:17804253-Interferon-gamma,
pubmed-meshheading:17804253-Luciferases, Firefly,
pubmed-meshheading:17804253-Oncorhynchus mykiss,
pubmed-meshheading:17804253-Promoter Regions, Genetic,
pubmed-meshheading:17804253-Salmon,
pubmed-meshheading:17804253-Sensitivity and Specificity,
pubmed-meshheading:17804253-Time Factors,
pubmed-meshheading:17804253-Togaviridae,
pubmed-meshheading:17804253-Togaviridae Infections
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| pubmed:year |
2007
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| pubmed:articleTitle |
A recombinant CHSE-214 cell line expressing an Mx1 promoter-reporter system responds to both interferon type I and type II from salmonids and represents a versatile tool to study the IFN-system in teleost fish.
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| pubmed:affiliation |
University of Tromsø, Norwegian College of Fishery Science, Department of Marine Biotechnology, N-9037 Tromsø, Norway. jorunn.jorgensen@nfh.uit.no
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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