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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1992-3-10
|
| pubmed:abstractText |
Rat insulin-like growth factor-I (IGF-I) mRNAs with different 5'-untranslated region/prepeptide coding sequences result from transcription initiation in one of two leader exons. While not altering the mature IGF-I coding sequence, these different leaders potentially encode two distinct IGF-I prepeptides, one of 48 amino acids (exon 1) and one of 32 amino acids (exon 2). Within exon 1, transcription initiation is dispersed (i.e. occurs over a approximately 350-basepair region), while within exon 2, it is highly localized. A fourth exon 1 start site, residing only approximately 30 basepairs from its 3' end, is suggested on the basis of RNase protection assays; its use would produce an mRNA encoding a third distinct IGF-I leader peptide of 22 amino acids. We have determined that during postnatal development, and as a result of insulinopenic diabetes and fasting, choice of transcription start sites within exon 1 in the liver is coordinately regulated, i.e. use of all start sites increased during development and decreased in the two catabolic states. Transcription initiation at the single major site within exon 2 was also reduced in diabetes and fasting. Insulin replacement therapy and refeeding restored the levels of all transcripts coordinately. During postnatal development, however, transcripts initiating within exon 2 exhibited a different developmental profile than did exon 1 transcripts, increasing especially at the onset of GH-dependent linear growth. In liver, therefore, negative regulation of exon 1 and exon 2 transcription start site usage occurs in catabolic states, while in development, differential regulation of exon 1 and exon 2 transcription start sites occurs.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antisense Elements (Genetics),
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Sorting Signals,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Probes
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0888-8809
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
5
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1677-86
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1779970-Aging,
pubmed-meshheading:1779970-Animals,
pubmed-meshheading:1779970-Antisense Elements (Genetics),
pubmed-meshheading:1779970-Base Sequence,
pubmed-meshheading:1779970-Chromosome Deletion,
pubmed-meshheading:1779970-Cloning, Molecular,
pubmed-meshheading:1779970-Diabetes Mellitus, Experimental,
pubmed-meshheading:1779970-Exons,
pubmed-meshheading:1779970-Fasting,
pubmed-meshheading:1779970-Fetus,
pubmed-meshheading:1779970-Gene Expression Regulation,
pubmed-meshheading:1779970-Gene Library,
pubmed-meshheading:1779970-Insulin-Like Growth Factor I,
pubmed-meshheading:1779970-Liver,
pubmed-meshheading:1779970-Male,
pubmed-meshheading:1779970-Molecular Sequence Data,
pubmed-meshheading:1779970-Oligodeoxyribonucleotides,
pubmed-meshheading:1779970-Protein Sorting Signals,
pubmed-meshheading:1779970-RNA, Messenger,
pubmed-meshheading:1779970-RNA Probes,
pubmed-meshheading:1779970-RNA Processing, Post-Transcriptional,
pubmed-meshheading:1779970-Rats,
pubmed-meshheading:1779970-Rats, Inbred Strains,
pubmed-meshheading:1779970-Restriction Mapping,
pubmed-meshheading:1779970-Transcription, Genetic
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Regulation of start site usage in the leader exons of the rat insulin-like growth factor-I gene by development, fasting, and diabetes.
|
| pubmed:affiliation |
Section on Molecular and Cellular Physiology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|