Linked Life Data

1779884

Source:http://linkedlifedata.com/resource/pubmed/id/1779884

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1992-3-9
pubmed:abstractText
In vivo dynamics of the interaction between leukocytes and the endothelium following direct activation of the white blood cells (WBCs), apart from possible endothelial cell activation, were studied in arterioles, capillaries, and venules of splanchnic tissue (rabbit omentum). WBCs were isolated using either density gradient or centrifugation techniques, labeled with fluorescent dyes, and exposed to physiological solutions with or without the chemoactivator N-formyl-methionyl-leucyl-phenlyalanine (FMLP). WBCs isolated using standard density gradient separation techniques rapidly disappeared from the circulating pool following a bolus injection and were sequestered in lung microvessels. The centrifugation technique produced cells that circulated for at least 60 min. WBCs directly activated with FMLP adhered to venular endothelium but not to arteriolar endothelium, suggesting that differences in hydrodynamics in the arteriolar and venular network or fundamental differences between arteriolar and venular endothelia may explain the lack of leukocyte-endothelium adhesion (LEA) in arterioles. WBCs pretreated with FMLP had significantly longer attachment times than nontreated cells, 13.4 and 2.5 sec respectively, which may be indicative of specific receptor chemistry. Similarities in the LEA attachment-detachment process for splanchnic tissue with that previously reported for lymphoid tissue suggest that a fundamental process of cell to cell interaction may exist in all tissues.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0026-2862
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
288-304
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Dynamics of leukocyte-endothelium interactions in the splanchnic microcirculation.
pubmed:affiliation
Department of Biology, College of Arts and Sciences, Seton Hall University, South Orange, New Jersey 07079.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't