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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2007-9-5
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pubmed:abstractText |
The MHC-encoded butyrophilin, BTN2A1, is a cell surface glycoprotein related to the extended family of B7 costimulatory molecules. BTN2A1 mRNA was expressed in most human tissues, but protein expression was significantly lower in leukocytes. An Ig-fusion protein of BTN2A1 bound to immature monocyte-derived dendritic cells. Binding diminished upon MoDC maturation and no binding was detected to Langerhans cells. Induction of the counterreceptor was IL-4 dependent and occurred early during dendritic cell differentiation. The interaction required the presence of Ca2+ and was mediated by high-mannose oligosaccharides. These properties matched DC-SIGN, a DC-specific HIV-1 entry receptor. This was confirmed by binding of soluble BTN2A1 to DC-SIGN-transfectants and its inhibition by a specific Ab. DC-SIGN bound to native BTN2A1 expressed on a range of tissues. However, BTN2A1 was not recognized on some normal cells such as HUVECs despite a similar expression level. The BTN2A1 of tumor cells such as HEK293T have more high-mannose moieties in comparison to HUVECs, and those high-mannose moieties are instrumental for binding to DC-SIGN. The data are consistent with tumor- or tissue-specific glycosylation of BTN2A1 governing recognition by DC-SIGN on immature monocyte-derived dendritic cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/DC-specific ICAM-3 grabbing...,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/butyrophilin
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
179
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3804-11
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pubmed:meshHeading |
pubmed-meshheading:17785817-Animals,
pubmed-meshheading:17785817-Antigens, CD80,
pubmed-meshheading:17785817-Cell Adhesion Molecules,
pubmed-meshheading:17785817-Cell Differentiation,
pubmed-meshheading:17785817-Cell Line, Tumor,
pubmed-meshheading:17785817-Cells, Cultured,
pubmed-meshheading:17785817-Dendritic Cells,
pubmed-meshheading:17785817-Gene Expression Regulation,
pubmed-meshheading:17785817-Glycosylation,
pubmed-meshheading:17785817-HeLa Cells,
pubmed-meshheading:17785817-Humans,
pubmed-meshheading:17785817-Jurkat Cells,
pubmed-meshheading:17785817-Langerhans Cells,
pubmed-meshheading:17785817-Lectins, C-Type,
pubmed-meshheading:17785817-Ligands,
pubmed-meshheading:17785817-Membrane Glycoproteins,
pubmed-meshheading:17785817-Mice,
pubmed-meshheading:17785817-Receptors, Cell Surface,
pubmed-meshheading:17785817-Recombinant Fusion Proteins,
pubmed-meshheading:17785817-Structural Homology, Protein,
pubmed-meshheading:17785817-Transfection
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pubmed:year |
2007
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pubmed:articleTitle |
The B7 homolog butyrophilin BTN2A1 is a novel ligand for DC-SIGN.
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pubmed:affiliation |
Department of Pathology, Division of Immunology, University of Cambridge, Cambridge, United Kingdom. gfjm2@cam.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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