Source:http://linkedlifedata.com/resource/pubmed/id/17784877
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2007-10-8
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| pubmed:abstractText |
The latent persistence of herpes simplex virus type 1 (HSV-1) in human trigeminal ganglia (TG) is accompanied by a chronic CD8 T-cell infiltrate. The focus of the current work was to look for HSV-1 transcription activity as a potential trigger of the immune response and to characterize the immune cell infiltrates by this feature. We combined in situ hybridization, laser cutting microscopy, and single cell RT-PCR to demonstrate the expression of the HSV-1 immediate early (IE) genes ICP0 and ICP4 in human trigeminal neurons. Using CDR3 spectratyping, we showed that the infiltrating T-cells are clonally expanded, indicating an antigen-driven immune response. Moreover, the persisting CD8+ T-cells had features of the memory effector phenotype. The voltage-gated potassium channel Kv1.3, a marker of chronic activated memory effector cells, and the chemokines CCL5 and CXCL10 were expressed by a subpopulation of infiltrating cells. The corresponding chemokine receptors CCR5 and CXCR3 were co-expressed on virtually all CD8 T-cells. In addition, T-cells expressed granzymes and perforin. In contrast to animal models of HSV-1 latency, hardly any FoxP3-positive regulatory T-cells were detected in human TG. Thus, HSV-1 IE genes are expressed in human TG and the infiltrating T-cells bear several characteristics that suggest viral antigenic stimulation.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
|
| pubmed:issn |
1015-6305
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| pubmed:author |
pubmed-author:ArbusowViktorV,
pubmed-author:BrandtThomasT,
pubmed-author:DerfussTobiasT,
pubmed-author:DornmairKlausK,
pubmed-author:EbeltKathleenK,
pubmed-author:HüfnerKatharinaK,
pubmed-author:HerbergerSimoneS,
pubmed-author:KnausHans-GuntherHG,
pubmed-author:MeinlEdgarE,
pubmed-author:SegererStephanS,
pubmed-author:SinicinaIngaI,
pubmed-author:SteinerIsraelI,
pubmed-author:StruppMichaelM,
pubmed-author:TheilDiethildeD
|
| pubmed:issnType |
Print
|
| pubmed:volume |
17
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
389-98
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| pubmed:meshHeading |
pubmed-meshheading:17784877-Adult,
pubmed-meshheading:17784877-Aged,
pubmed-meshheading:17784877-Aged, 80 and over,
pubmed-meshheading:17784877-CD8-Positive T-Lymphocytes,
pubmed-meshheading:17784877-Chemokines,
pubmed-meshheading:17784877-Chemotaxis, Leukocyte,
pubmed-meshheading:17784877-Clone Cells,
pubmed-meshheading:17784877-Female,
pubmed-meshheading:17784877-Gene Expression Regulation, Viral,
pubmed-meshheading:17784877-Genes, Immediate-Early,
pubmed-meshheading:17784877-Genes, Viral,
pubmed-meshheading:17784877-Herpes Simplex,
pubmed-meshheading:17784877-Herpesvirus 1, Human,
pubmed-meshheading:17784877-Humans,
pubmed-meshheading:17784877-Immunologic Memory,
pubmed-meshheading:17784877-Kv1.3 Potassium Channel,
pubmed-meshheading:17784877-Male,
pubmed-meshheading:17784877-Middle Aged,
pubmed-meshheading:17784877-Neurons, Afferent,
pubmed-meshheading:17784877-Phenotype,
pubmed-meshheading:17784877-Receptors, Chemokine,
pubmed-meshheading:17784877-T-Lymphocytes,
pubmed-meshheading:17784877-Trigeminal Ganglion,
pubmed-meshheading:17784877-Virus Latency
|
| pubmed:year |
2007
|
| pubmed:articleTitle |
Presence of HSV-1 immediate early genes and clonally expanded T-cells with a memory effector phenotype in human trigeminal ganglia.
|
| pubmed:affiliation |
Institute of Clinical Neuroimmunology, University Clinic, Munich, Germany. tsderfus@neuro.mpg.de
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|