Linked Life Data

17766682

Source:http://linkedlifedata.com/resource/pubmed/id/17766682

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2007-11-16
pubmed:abstractText
Administration of high-dose intact human immunoglobulin (IH-Ig) has been applied to treat a variety of inflammatory and autoimmune diseases, and is expected to have beneficial effects on human fecundity. In the present study, we investigated whether Ig had anti-resorption effects using polyinosinic-polycytidylic acid sodium salt [poly (I:C)]-induced enhancement of fetal resorption in the mating of CBA/J x DBA/2J resorption-prone mouse model. Furthermore, we investigated the mechanism of the effect by examining the mRNA expression of interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, IL-10, IL-4 and TGF-beta(1) in spleens and placentas from the resorption-prone model treated with IH-Ig, by reverse transcription (RT)-polymerase chain reaction (PCR). Administration of high-dose IH-Ig significantly reduced the fetal resorption rate from 55% to 10%. This anti-resorption effect, however, was not detected in mice administered with Fab fragments of human Ig. We then performed adoptive transfer experiments to examine whether cellular components could transfer the effect. A remarkable anti-resorption effect was seen in poly (I:C)-injected pregnant recipients transferred with spleen cells from IH-Ig-treated donor mice. The RT-PCR study showed that IH-Ig reduced the expression of IFN-gamma and TNF-alpha mRNA in placentas of poly (I:C)-injected pregnant mice. The present findings demonstrate that intact Ig, particularly its Fc portion, possesses anti-resorption activity. The effect might be attributed to the suppressed production of pro-inflammatory cytokines at the maternofetal interface.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1460-2407
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
807-14
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:17766682-Animals, pubmed-meshheading:17766682-Female, pubmed-meshheading:17766682-Fetal Resorption, pubmed-meshheading:17766682-Flow Cytometry, pubmed-meshheading:17766682-Fluoresceins, pubmed-meshheading:17766682-Gene Expression, pubmed-meshheading:17766682-Immunoglobulin G, pubmed-meshheading:17766682-Immunoglobulins, pubmed-meshheading:17766682-Interferon-gamma, pubmed-meshheading:17766682-Interleukin-10, pubmed-meshheading:17766682-Interleukin-4, pubmed-meshheading:17766682-Male, pubmed-meshheading:17766682-Mice, pubmed-meshheading:17766682-Placenta, pubmed-meshheading:17766682-Pregnancy, pubmed-meshheading:17766682-RNA, Messenger, pubmed-meshheading:17766682-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:17766682-Spleen, pubmed-meshheading:17766682-Transforming Growth Factor beta, pubmed-meshheading:17766682-Tumor Necrosis Factor-alpha
pubmed:year
2007
pubmed:articleTitle
Administration of high-dose intact immunoglobulin has an anti-resorption effect in a mouse model of reproductive failure.
pubmed:affiliation
Division of Immunobiology, Research Section of Pathophysiology, Institute for Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo, Hokkaido, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't