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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2007-8-31
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pubmed:abstractText |
We were interested whether PKC alpha, delta, epsilon or zeta is the isoform actually employed in the activation of hypertonicity-induced cation channels (HICCs) in primary cultures of rat hepatocytes. Quantitative SDS-page and Western-blot experiments revealed that PKC alpha, delta and epsilon were stimulated by Indolactam V (as a DAG substitute for activation of c and nPKCs) but that only PKC delta and epsilon did respond to hypertonic stress. Furthermore, chelation of intracellular Ca(++) by BAPTA-AM did not alter HICC activation in cable-analysis experiments whereas Indolactam V as well as V8 (an Indolactam derivative specific for PKC delta and epsilon) activated HICC currents under isotonic conditions. Finally, by use of Rottlerin (as an inhibitor exhibiting a slight preference for PKC delta over epsilon) PKC epsilon could be identified as the most likely isoform responsible for the activation of the HICC.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1015-8987
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pubmed:author |
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pubmed:copyrightInfo |
Copyright (c) 2007 S. Karger AG, Basel.
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pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
397-404
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:17762167-Acetophenones,
pubmed-meshheading:17762167-Animals,
pubmed-meshheading:17762167-Benzopyrans,
pubmed-meshheading:17762167-Cells, Cultured,
pubmed-meshheading:17762167-Enzyme Activation,
pubmed-meshheading:17762167-Hepatocytes,
pubmed-meshheading:17762167-Indoles,
pubmed-meshheading:17762167-Ion Channels,
pubmed-meshheading:17762167-Isoenzymes,
pubmed-meshheading:17762167-Lactams,
pubmed-meshheading:17762167-Protein Kinase C-epsilon,
pubmed-meshheading:17762167-Rats
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pubmed:year |
2007
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pubmed:articleTitle |
The epsilon-isoform of PKC mediates the hypertonic activation of cation channels in confluent monolayers of rat hepatocytes.
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pubmed:affiliation |
Department of Systemic Cell Biology, Max-Planck-Institute for Molecular Physiology, Dortmund, Germany.
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pubmed:publicationType |
Journal Article
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