Linked Life Data

1774231

Source:http://linkedlifedata.com/resource/pubmed/id/1774231

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1992-3-3
pubmed:abstractText
A simple chemical confirmatory technique has been developed for domoic acid, a neurotoxic amino acid of marine origin. After extraction with water-methanol, the domoic acid-containing extract is analysed directly by reversed-phase liquid chromatography with UV absorption detection at 242 nm. For confirmation of positive results an aliquot of the extract is evaporated to dryness and reacted with butyl isothiocyanate to form a thiourea derivative which elutes later than underivatized domoic acid. No additional sample cleanup is required in order to carry out the derivatization for conformation of domoic acid at the Canadian 20 micrograms/g guideline level in shellfish. In mussel extract, domoic acid was converted to the thiourea derivative with a yield of 86-91% compared to a pure standard carried through the same reaction. The detection limit for the derivative was about 5-10 micrograms/g of equivalent domoic acid in extracts of mussels, clams or oysters.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
550
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
595-601
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Confirmation of domoic acid in shellfish using butyl isothiocyanate and reversed-phase liquid chromatography.
pubmed:affiliation
Food Research Division, Health Protection Branch, Ottawa, Ontario, Canada.
pubmed:publicationType
Journal Article