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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1992-3-2
|
| pubmed:abstractText |
The major extracellular polysaccharide (glucuronoxylomannan, GXM) from six strains of Cryptococcus neoformans serotype B was characterized by gas-liquid chromatography (g.l.c.), g.l.c.-mass spectrometry (g.l.c.-m.s.), and nuclear magnetic resonance (n.m.r.) spectroscopy. Ultrasonic irradiation (u.i.) was used to reduce the mol.wt. of native GXM from 9.75 x 10(5) to 1.15 x 10(5) without apparent change in its composition (GXM-S). The Xylp:Manp:GlcpA molar ratio of the GXM and GXM-S from the six strains of C. neoformans serotype B is approximately 3.5:3.0:0.6. GXM-S was O-deacetylated (GXM-D) by treatment with NH4OH. The 13C-n.m.r. analysis of GXM-D gave spectra that served as characteristic fingerprints of the structure and also facilitated the assignment of the anomeric carbon resonances to specific structural moieties present in GXM-D. The GXM-D from each serotype B strain was found to be similar by 13C-n.m.r. spectroscopy. The structure contains a linear (1----3)-alpha-D-Manp backbone substituted with 2-O-beta-GlcpA and 2-O-beta-Xylp. beta-Xylp is also O-4 linked to the Manp substituted with GlcpA. In addition, a model for the disposition of the Xylp and GlcpA side chain substituents along the mannopyranan backbone is proposed, based upon results from the combination of g.l.c.-m.s. and 13C-n.m.r. spectroscopy.
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| pubmed:grant | |
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carbohydrates,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Isotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Lithium,
http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/glucuronoxylomannan
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0008-6215
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
2
|
| pubmed:volume |
211
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
103-16
|
| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:1773425-Carbohydrate Sequence,
pubmed-meshheading:1773425-Carbohydrates,
pubmed-meshheading:1773425-Carbon Isotopes,
pubmed-meshheading:1773425-Chromatography, Gas,
pubmed-meshheading:1773425-Chromatography, Gel,
pubmed-meshheading:1773425-Chromatography, Ion Exchange,
pubmed-meshheading:1773425-Cryptococcus neoformans,
pubmed-meshheading:1773425-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:1773425-Lithium,
pubmed-meshheading:1773425-Magnetic Resonance Spectroscopy,
pubmed-meshheading:1773425-Molecular Sequence Data,
pubmed-meshheading:1773425-Molecular Weight,
pubmed-meshheading:1773425-Oxidation-Reduction,
pubmed-meshheading:1773425-Polysaccharides,
pubmed-meshheading:1773425-Polysaccharides, Bacterial,
pubmed-meshheading:1773425-Serotyping,
pubmed-meshheading:1773425-Sonication
|
| pubmed:year |
1991
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| pubmed:articleTitle |
Glucuronoxylomannan of Cryptococcus neoformans serotype B: structural analysis by gas-liquid chromatography-mass spectrometry and 13C-nuclear magnetic resonance spectroscopy.
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| pubmed:affiliation |
Department of Chemistry, Georgia State University, Atlanta 30303.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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