|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1976-7-6
|
|
pubmed:abstractText |
1. Tubulin is not an adenosine-3':5'-monophosphate-dependent (cyclic-AMP-dependent) protein kinase. Both entities have been clearly separated by sucrose gradient ultracentrifugation. With a tubulin preparation obtained by the polymerization-depolymerization technique protein kinase had a sedimentation coefficient of 8.7 S whereas tubulin sedimented with 6.4 S. After preincubation with both cyclic AMP and histone the kinase dissociated into its catalytic subunit with a sedimentation coefficient of 3.4 S. 2. Tubulin prepared by the polymerization-depolymerization technique was neither phosphorylated in vivo nor in vitro. On the contrary if this preparation was further purified by the Weisenberg's procedure (DEAE-Sephadex batch absorption) before incubation with [gamma-32 P]ATP, phosphorylation occurred. Thus, phosphorylation depended on the method used to purify tubulin i.e. was likely to an an artefact.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0014-2956
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
62
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
539-49
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:177284-Brain,
pubmed-meshheading:177284-Centrifugation, Density Gradient,
pubmed-meshheading:177284-Cyclic AMP,
pubmed-meshheading:177284-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:177284-Enzyme Activation,
pubmed-meshheading:177284-Fluorides,
pubmed-meshheading:177284-Kinetics,
pubmed-meshheading:177284-Macromolecular Substances,
pubmed-meshheading:177284-Microtubules,
pubmed-meshheading:177284-Molecular Weight,
pubmed-meshheading:177284-Nerve Tissue Proteins,
pubmed-meshheading:177284-Organ Specificity,
pubmed-meshheading:177284-Phosphoproteins,
pubmed-meshheading:177284-Protein Kinases,
pubmed-meshheading:177284-Thyroid Gland
|
|
pubmed:year |
1976
|
|
pubmed:articleTitle |
Phosphorylation of microtubule-associated proteins.
|
|
pubmed:publicationType |
Journal Article
|
