Source:http://linkedlifedata.com/resource/pubmed/id/17723265
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2007-9-21
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pubmed:abstractText |
Subarachnoid hemorrhage (SAH) initiates a series of cellular and molecular events, some of which involve a mitogen activated protein kinase, c-jun N-terminal kinase (JNK). However, precise details regarding activation of c-jun in the vessel wall after SAH largely remain to be elucidated. In this study, we therefore investigated the localization and time-dependent expression of c-jun in the rat basilar artery after SAH in a rat single-hemorrhage model featuring infusion of autologous arterial blood. Basilar arteries were obtained at 2, 6 and 12h and 1, 2, 4 and 7 days after SAH, as well as from controls. Western blot analysis with c-jun, phosphorylated c-jun at Ser(63), and actin antibodies revealed that c-jun was immediately phosphorylated at Ser(63) within 2h, thereafter gradually becoming dephosphorylated, while total c-jun and actin levels remained almost unchanged. Immunohistochemistry demonstrated phosphorylation of c-jun at Ser(63) to occur in smooth muscle cells of the basilar artery 2h after SAH. These results indicate that c-jun is activated in the basilar artery immediately after the onset of SAH, presumably resulting in transcription of immediate early genes and smooth muscle cell proliferation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0304-3940
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
424
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
175-8
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pubmed:meshHeading |
pubmed-meshheading:17723265-Animals,
pubmed-meshheading:17723265-Basilar Artery,
pubmed-meshheading:17723265-Disease Models, Animal,
pubmed-meshheading:17723265-Enzyme Activation,
pubmed-meshheading:17723265-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:17723265-Male,
pubmed-meshheading:17723265-Phosphorylation,
pubmed-meshheading:17723265-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:17723265-Rats,
pubmed-meshheading:17723265-Rats, Sprague-Dawley,
pubmed-meshheading:17723265-Serine,
pubmed-meshheading:17723265-Subarachnoid Hemorrhage,
pubmed-meshheading:17723265-Time Factors
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pubmed:year |
2007
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pubmed:articleTitle |
Activation of c-jun in the rat basilar artery after subarachnoid hemorrhage.
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pubmed:affiliation |
Department of Neurological Surgery, Aichi Medical University, 21 Karimata Yazako, Nagakute, Aichi-gun 480-1195, Japan.
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pubmed:publicationType |
Journal Article
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