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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
43
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pubmed:dateCreated |
2007-10-22
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pubmed:abstractText |
Cripto-1 (CR-1) is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein that has been shown to play an important role in embryogenesis and cellular transformation. CR-1 is reported to function as a membrane-bound co-receptor and as a soluble ligand. Although a number of studies implicate the role of CR-1 as a soluble ligand in tumor progression, it is unclear how transition from the membrane-bound to the soluble form is physiologically regulated and whether differences in biological activity exist between these forms. Here, we demonstrate that CR-1 protein is secreted from tumor cells into the conditioned medium after treatment with serum, epidermal growth factor, or lysophosphatidic acid, and this soluble form of CR-1 exhibits the ability to promote endothelial cell migration as a paracrine chemoattractant. On the other hand, membrane-bound CR-1 can stimulate endothelial cell sprouting through direct cell-cell interaction. Shedding of CR-1 occurs at the GPI-anchorage site by the activity of GPI-phospholipase D (GPI-PLD), because CR-1 shedding was suppressed by siRNA knockdown of GPI-PLD and enhanced by overexpression of GPI-PLD. These findings describe a novel molecular mechanism of CR-1 shedding, which may contribute to endothelial cell migration and possibly tumor angiogenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DAPI,
http://linkedlifedata.com/resource/pubmed/chemical/Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/GPI-Linked Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosylphosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phalloidine,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase D,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Rhodamines,
http://linkedlifedata.com/resource/pubmed/chemical/TDGF1 protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:BaillyVeroniqueV,
pubmed-author:BiancoCaterinaC,
pubmed-author:GonzalesMonicaM,
pubmed-author:HamadaShinS,
pubmed-author:MancinoMarioM,
pubmed-author:MiatkowskiKonradK,
pubmed-author:MoWenjunW,
pubmed-author:SalomonDavid SDS,
pubmed-author:SanicolaMicheleM,
pubmed-author:SenoMasaharuM,
pubmed-author:StrizziLuigiL,
pubmed-author:WatanabeKazuhideK,
pubmed-author:WenDingyiD
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
282
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
31643-55
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:17720976-Adenocarcinoma,
pubmed-meshheading:17720976-Animals,
pubmed-meshheading:17720976-COS Cells,
pubmed-meshheading:17720976-Cell Line,
pubmed-meshheading:17720976-Cell Line, Tumor,
pubmed-meshheading:17720976-Cell Movement,
pubmed-meshheading:17720976-Cercopithecus aethiops,
pubmed-meshheading:17720976-Coculture Techniques,
pubmed-meshheading:17720976-Colonic Neoplasms,
pubmed-meshheading:17720976-Dogs,
pubmed-meshheading:17720976-Endothelial Cells,
pubmed-meshheading:17720976-Endothelium, Vascular,
pubmed-meshheading:17720976-Epidermal Growth Factor,
pubmed-meshheading:17720976-Fluorescent Dyes,
pubmed-meshheading:17720976-GPI-Linked Proteins,
pubmed-meshheading:17720976-Glycosylphosphatidylinositols,
pubmed-meshheading:17720976-Growth Substances,
pubmed-meshheading:17720976-Humans,
pubmed-meshheading:17720976-Indoles,
pubmed-meshheading:17720976-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:17720976-Kidney,
pubmed-meshheading:17720976-Mass Spectrometry,
pubmed-meshheading:17720976-Membrane Glycoproteins,
pubmed-meshheading:17720976-Neoplasm Proteins,
pubmed-meshheading:17720976-Phalloidine,
pubmed-meshheading:17720976-Phospholipase D,
pubmed-meshheading:17720976-RNA, Small Interfering,
pubmed-meshheading:17720976-Rhodamines,
pubmed-meshheading:17720976-Umbilical Veins
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pubmed:year |
2007
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pubmed:articleTitle |
Growth factor induction of Cripto-1 shedding by glycosylphosphatidylinositol-phospholipase D and enhancement of endothelial cell migration.
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pubmed:affiliation |
Tumor Growth Factor Section, Mammary Biology & Tumorigenesis Laboratory, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Intramural
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