Source:http://linkedlifedata.com/resource/pubmed/id/17715390
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
13
|
| pubmed:dateCreated |
2007-12-6
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| pubmed:abstractText |
To address the mechanism by which the human globin genes are activated during erythropoiesis, we have used a tiled microarray to analyze the pattern of transcription factor binding and associated histone modifications across the telomeric region of human chromosome 16 in primary erythroid and nonerythroid cells. This 220-kb region includes the alpha globin genes and 9 widely expressed genes flanking the alpha globin locus. This un-biased, comprehensive analysis of transcription factor binding and histone modifications (acetylation and methylation) described here not only identified all known cis-acting regulatory elements in the human alpha globin cluster but also demonstrated that there are no additional erythroid-specific regulatory elements in the 220-kb region tested. In addition, the pattern of histone modification distinguished promoter elements from potential enhancer elements across this region. Finally, comparison of the human and mouse orthologous regions in a unique mouse model, with both regions coexpressed in the same animal, showed significant differences that may explain how these 2 clusters are regulated differently in vivo.
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| pubmed:grant | |
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0006-4971
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| pubmed:author |
pubmed-author:AnguitaEduardoE,
pubmed-author:De GobbiMarcoM,
pubmed-author:DunhamIanI,
pubmed-author:GibbonsRichard JRJ,
pubmed-author:HiggsDouglas RDR,
pubmed-author:HughesJimJ,
pubmed-author:KochChristoph MCM,
pubmed-author:SharpeJacqueline AJA,
pubmed-author:Sloane-StanleyJacqueline AJA,
pubmed-author:WoodWilliam GWG
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| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
110
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4503-10
|
| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:17715390-Acetylation,
pubmed-meshheading:17715390-Animals,
pubmed-meshheading:17715390-Cells, Cultured,
pubmed-meshheading:17715390-Chromosomes, Human, Pair 16,
pubmed-meshheading:17715390-Enhancer Elements, Genetic,
pubmed-meshheading:17715390-Erythroblasts,
pubmed-meshheading:17715390-Gene Expression Regulation,
pubmed-meshheading:17715390-Globins,
pubmed-meshheading:17715390-Histones,
pubmed-meshheading:17715390-Humans,
pubmed-meshheading:17715390-K562 Cells,
pubmed-meshheading:17715390-Methylation,
pubmed-meshheading:17715390-Mice,
pubmed-meshheading:17715390-Promoter Regions, Genetic,
pubmed-meshheading:17715390-T-Lymphocytes,
pubmed-meshheading:17715390-Telomere,
pubmed-meshheading:17715390-Transcription, Genetic,
pubmed-meshheading:17715390-Transcription Factors
|
| pubmed:year |
2007
|
| pubmed:articleTitle |
Tissue-specific histone modification and transcription factor binding in alpha globin gene expression.
|
| pubmed:affiliation |
Medical Research Council, Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, Oxford University, Oxford, UK.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|