Source:http://linkedlifedata.com/resource/pubmed/id/17712332
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
|
pubmed:dateCreated |
2007-10-19
|
pubmed:abstractText |
By the time we are adolescents most of us have been in contact with several of the >50 human adenovirus (HAd) serotypes. These common subclinical infections lead to an efficient anti-adenovirus cross-reacting adaptive immunity. During gene therapy, the ubiquitous anti-adenovirus humoral response and complement activation will modify and dictate vector biodistribution, as well as the response to the virion and transgene(s). In this study, we assayed the interactions of a xenogenic adenovirus derived from canine serotype 2 (CAV-2) with naturally occurring human antibodies (Abs) and the complement system. In our cohort, we found class G immunoglobulins (Igs) that recognized the intact CAV-2 virion and the external virion proteins. However, the majority of donors had low or no neutralizing Abs, class A, or class M Igs. Purified anti-HAd serotype 5 Abs also recognized CAV-2 virion proteins. In addition, in spite of the presence of anti-CAV-2 IgGs, CAV-2 poorly activated the classical and alternative complement cascades. This atypical response was due to a block upstream of the component 3 (C3) convertase and interplay between the component 1 (C1) inhibitor, the C1q-C1r2-C1s2 complex and CAV-2. Our data demonstrate that some xenogenic adenovirus vectors, like CAV-2, could lead to notably different outcomes following systemic delivery.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Ribosome Inactivating Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sambucus nigra lectins
|
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
1525-0016
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
15
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1998-2007
|
pubmed:meshHeading |
pubmed-meshheading:17712332-Adenoviridae,
pubmed-meshheading:17712332-Antibody Formation,
pubmed-meshheading:17712332-Blood Proteins,
pubmed-meshheading:17712332-Cross Reactions,
pubmed-meshheading:17712332-Genetic Vectors,
pubmed-meshheading:17712332-Humans,
pubmed-meshheading:17712332-Immunogenetics,
pubmed-meshheading:17712332-Immunoglobulin G,
pubmed-meshheading:17712332-Plant Lectins,
pubmed-meshheading:17712332-Ribosome Inactivating Proteins,
pubmed-meshheading:17712332-Signal Transduction,
pubmed-meshheading:17712332-Transduction, Genetic
|
pubmed:year |
2007
|
pubmed:articleTitle |
Interactions between human plasma components and a xenogenic adenovirus vector: reduced immunogenicity during gene transfer.
|
pubmed:affiliation |
Institut de Génétique Moléculaire de Montpellier, Montpellier, France.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|