Linked Life Data

17709135

Source:http://linkedlifedata.com/resource/pubmed/id/17709135

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pubmed-article:17709135pubmed:dateCreated2007-9-10lld:pubmed
pubmed-article:17709135pubmed:abstractTextTissue engineering seeks to provide regenerated tissue architectures in vitro but has not yet successfully created thick, highly vascularized, multi-functional tissues replicating native structure. We describe a novel method to fabricate pre-vascularized tissue equivalents using multi-layered cultures combining micro-patterned endothelial cells as vascular pre-cursors with fibroblast monolayer sheets as tissue matrix. Stratified tissue equivalents are constructed by alternately layering fibroblast monolayer sheets with patterned endothelial cell sheets harvested from newly developed thermo-responsive micro-patterned surfaces alternating 20 microm-wide cell-adhesive lanes with 60 microm non-adhesive zones. Cell culture substrates covalently grafted with different thermo-responsive polymers permit spatial switching of cell adhesion and detachment using applied small temperature changes. Endothelial cell patterning fidelity was maintained within the multi-layer tissue constructs after assembly, leading to self-organization into microvascular-like networks after 5-day tissue culture. This novel technique holds promise for the study of cell-cell communications and angiogenesis in reconstructed, three-dimensional environments as well as for the fabrication of tissues with complex, multicellular architecture.lld:pubmed
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pubmed-article:17709135pubmed:authorpubmed-author:KobayashiJunJlld:pubmed
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pubmed-article:17709135pubmed:year2007lld:pubmed
pubmed-article:17709135pubmed:articleTitleCellular control of tissue architectures using a three-dimensional tissue fabrication technique.lld:pubmed
pubmed-article:17709135pubmed:affiliationGraduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, Japan.lld:pubmed
pubmed-article:17709135pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:17709135pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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