Source:http://linkedlifedata.com/resource/pubmed/id/17708788
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2007-8-21
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| pubmed:abstractText |
To study the FLT3 gene expression and its internal tandem duplication in hematologic malignancies and its clinical significance, polymerase chain reaction (PCR) and DNA sequencing were used to detect the FLT3/ITD mutation in blast cells of bone marrow from 86 patients with hematologic malignancies, including 32 cases of acute myeoloid leukemia (AML), 18 cases of acute lymphoblastic leukemia (ALL), 2 cases of acute hybrid leukemia (AHL), 12 cases of myelodysplastic syndromes (MDS), 10 cases of chronic myelogenous leukemia (CML), 3 cases of non-Hodgkin's lymphoma (NHL) and 9 cases of multiple myeloma (MM). The resultes showed that the expression of FLT3/ITD gene could be detected in 5 of 32 (15.6%) AML patients, including 1/7 of M(3), 1/10 of M(4) and 3/10 of M(5). More FLT3 aberrations were found in AML-M(5). No FLT3/ITD was found in 18 cases of ALL, in 2 cases of AHL, in 12 cases of MDS and in 10 cases of CML. No FLT3 was found in 3 cases of NHL and in 9 cases of MM. Sequence analysis in 2 case with abnormal PCR electrophoretic patterns revealed that the ITDs were located within exon 14 from 27 to 63 bp, which was a simple tandem duplication, and did not altered the reading frame. FLT3/ITD was associated with a higher peripheral blood white cell count (p < 0.01), higher percentage of bone marrow blast cells (p < 0.01) and lower complete mission rate. It is concluded that more FLT3/ITD mutation occurs in AML-M(5) patients. Sequence of the mutants is in frame mutation. FLT3/ITD mutation is associated with higher peripheral blood white cell count, higher percentage of bone marrow blast cells and lower complete remission rate, FIT3/IID gene mutation may be used to predict prognosis of patients with AML.
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| pubmed:language |
chi
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
1009-2137
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
15
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
709-13
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| pubmed:meshHeading |
pubmed-meshheading:17708788-Adolescent,
pubmed-meshheading:17708788-Adult,
pubmed-meshheading:17708788-Aged,
pubmed-meshheading:17708788-Aged, 80 and over,
pubmed-meshheading:17708788-Amino Acid Sequence,
pubmed-meshheading:17708788-Base Sequence,
pubmed-meshheading:17708788-Child,
pubmed-meshheading:17708788-Female,
pubmed-meshheading:17708788-Gene Duplication,
pubmed-meshheading:17708788-Hematologic Neoplasms,
pubmed-meshheading:17708788-Humans,
pubmed-meshheading:17708788-Male,
pubmed-meshheading:17708788-Middle Aged,
pubmed-meshheading:17708788-Molecular Sequence Data,
pubmed-meshheading:17708788-Mutation,
pubmed-meshheading:17708788-Prognosis,
pubmed-meshheading:17708788-Tandem Repeat Sequences,
pubmed-meshheading:17708788-Young Adult,
pubmed-meshheading:17708788-fms-Like Tyrosine Kinase 3
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| pubmed:year |
2007
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| pubmed:articleTitle |
[Detection of FLT3 gene mutation in hematologic malignancies and its clinical significance].
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| pubmed:affiliation |
Department of Microbiology, Qingdao University Medical College, Qingdao 266021, China.
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| pubmed:publicationType |
Journal Article,
English Abstract
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