Source:http://linkedlifedata.com/resource/pubmed/id/17708553
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
|
| pubmed:dateCreated |
2007-10-31
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| pubmed:abstractText |
The association between total and integrated HPV-33 DNA loads and high-grade squamous intraepithelial lesions (HSIL) of the uterine cervix was investigated. Of 5,347 women recruited in 4 studies, 89 (64 without SIL, 7 low-grade SIL (LSIL), 15 HSIL, 3 unknown grade) were infected by HPV-33. HPV-33 E6, HPV-33 E2 and beta-globin DNA were measured with real-time PCR that allowed to assess total (E6), episomal (E2) and integrated (E6-E2) HPV-33 viral loads. HPV-33 E6/E2 ratios >/=>/=2.0 suggesting the presence of integrated HPV-33 were obtained for 28.6% (n = 18) of women without SIL and 21.4% (n = 3) of women with HSIL (p = 0.74). Although median viral loads were similar, there was a trend toward having a greater proportion of women with HSIL in the fourth quartile (>/=>/=10(6.69) copies/mug DNA) of total HPV-33 viral loads compared to normal women. Controlling for age, site, ethnicity and LCR polymorphism by logistic regression, HPV-33 total loads in the fourth quartile {odds ratio (OR) 4.5 [95% confidence interval (CI) 1.2-17.3]; p = 0.03} and episomal loads in the fourth quartile (>/=>/=10(6.64) copies/mug DNA) [OR 3.9 (95% CI 1.1-13.2); p = 0.05] but not integrated HPV-33 load in the fourth quartile [OR 1.0 (95% CI 0.3-3.3); p = 0.50] were associated with HSIL. Controlling for age, study site and SIL grade, HPV-33 episomal load [OR 0.2 (95% CI 0.1-0.5), p = 0.0004] was associated with the presence of HPV-33 integration. High episomal loads in HSIL and the presence of integration in women without SIL are likely to weaken the usefulness of HPV load of integrated forms in clinical practice.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
1097-0215
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| pubmed:author |
pubmed-author:CoutléeFrançoisF,
pubmed-author:FerenczyAlex SAS,
pubmed-author:FrancoEduardo LEL,
pubmed-author:GagnonSimonS,
pubmed-author:KhouadriSorayaS,
pubmed-author:KoushikAnitaA,
pubmed-author:MatlashewskiGregG,
pubmed-author:RichardsonHarrietH,
pubmed-author:RogerMichelM,
pubmed-author:VillaLuisa LLL
|
| pubmed:copyrightInfo |
(c) 2007 Wiley-Liss, Inc.
|
| pubmed:issnType |
Electronic
|
| pubmed:day |
15
|
| pubmed:volume |
121
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2674-81
|
| pubmed:meshHeading |
pubmed-meshheading:17708553-Adult,
pubmed-meshheading:17708553-Carcinoma, Squamous Cell,
pubmed-meshheading:17708553-Case-Control Studies,
pubmed-meshheading:17708553-Cohort Studies,
pubmed-meshheading:17708553-Cytopathogenic Effect, Viral,
pubmed-meshheading:17708553-DNA, Viral,
pubmed-meshheading:17708553-DNA Probes, HPV,
pubmed-meshheading:17708553-Female,
pubmed-meshheading:17708553-Humans,
pubmed-meshheading:17708553-Logistic Models,
pubmed-meshheading:17708553-Middle Aged,
pubmed-meshheading:17708553-Odds Ratio,
pubmed-meshheading:17708553-Papillomaviridae,
pubmed-meshheading:17708553-Papillomavirus Infections,
pubmed-meshheading:17708553-Plasmids,
pubmed-meshheading:17708553-Polymerase Chain Reaction,
pubmed-meshheading:17708553-Polymorphism, Genetic,
pubmed-meshheading:17708553-Tumor Virus Infections,
pubmed-meshheading:17708553-Uterine Cervical Dysplasia,
pubmed-meshheading:17708553-Uterine Cervical Neoplasms,
pubmed-meshheading:17708553-Viral Envelope Proteins,
pubmed-meshheading:17708553-Viral Load
|
| pubmed:year |
2007
|
| pubmed:articleTitle |
Viral load of episomal and integrated forms of human papillomavirus type 33 in high-grade squamous intraepithelial lesions of the uterine cervix.
|
| pubmed:affiliation |
Laboratoire de Virologie Moléculaire du Centre de Recherche, Centre Hospitalier de l'Université de Montréal, Université de Montréal, Québec, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Multicenter Study,
Research Support, N.I.H., Extramural
|