Linked Life Data

17706391

Source:http://linkedlifedata.com/resource/pubmed/id/17706391

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-10-9
pubmed:abstractText
Several liquid chromatographic (LC) methods have been described for the analysis of gentamicin. LC combined with pulsed electrochemical detection (LC-PED) or evaporative light scattering detection (LC-ELSD) was found to be the most suitable technique. A first method, previously developed by Adams et al. used a poly(styrene-divinyl benzene) stationary phase with a mobile phase containing sodium sulphate, sodium-1-octanesulphonate, tetrahydrofuran, 0.2M phosphate buffer (pH 3) and water. However, the polymer columns show low efficiency, which also leads to poor sensitivity. So, recently the use of newer conventional C18 columns was further investigated. Improved separation was obtained using a Supelcosil LC-18-DB column with an adapted mobile phase. Another method derived from a company method was checked by using a Gemini column and a mobile phase containing an aqueous solution of trifluoroacetic acid (TFA) and pentafluoropropionic acid (PFPA) adjusted to pH 2.6 with sodium hydroxide (NaOH). This method was transferred to ELSD by replacing the non-volatile NaOH with volatile ammonium hydroxide solution. A volatile method, which was originally developed for ELSD using an aqueous solution of 50mM TFA and gradient elution with methanol, was also combined with PED. In this study, these methods were compared with regard to their selectivity, sensitivity and ease of use.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0731-7085
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
257-62
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Comparison of liquid chromatographic methods with direct detection for the analysis of gentamicin.
pubmed:affiliation
Laboratorium voor Farmaceutische Analyse, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, O&N2, PB 923, Herestraat 49, B-3000 Leuven, Belgium.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't