Source:http://linkedlifedata.com/resource/pubmed/id/17704754
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
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| pubmed:dateCreated |
2007-10-11
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| pubmed:abstractText |
DNA vaccine and dendritic cells (DCs)-based vaccine have emerged as promising strategies for cancer immunotherapy. Fms-like tyrosine kinase 3-ligand (Flt3L) and granulocyte-macrophage-colony-stimulating factor (GM-CSF) have been exploited for the expansion of DC. It was reported previously that combination of plasmid encoding GM-CSF with HER2/neu DNA vaccine induced predominantly CD4(+) T-cell-mediated antitumor immune response. In this study, we investigated the modulation of immune responses by murine Flt3L and GM-CSF, which acted as genetic adjuvants in the forms of bicistronic (pFLAG) and monocistronic (pFL and pGM) plasmids for HER2/neu DNA vaccine (pN-neu). Coexpression of Flt3L and GM-CSF significantly enhanced maturation and antigen-presentation abilities of splenic DC. Increased numbers of infiltrating DC at the immunization site, higher interferon-gamma production, and enhanced cytolytic activities by splenocytes were prominent in mice vaccinated with pN-neu in conjunction with pFLAG. Importantly, a potent CD8(+) T-cell-mediated antitumor immunity against bladder tumors naturally overexpressing HER2/neu was induced in the vaccinated mice. Collectively, our results indicate that murine Flt3L and GM-CSF genes coexpressed by a bicistronic plasmid modulate the class of immune responses and may be superior to those codelivered by two separate monocistronic plasmids as the genetic adjuvants for HER2/neu DNA vaccine.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cancer Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, erbB-2,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA,
http://linkedlifedata.com/resource/pubmed/chemical/flt3 ligand protein
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0929-1903
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
14
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
904-17
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:17704754-Animals,
pubmed-meshheading:17704754-Antigen Presentation,
pubmed-meshheading:17704754-CD4-Positive T-Lymphocytes,
pubmed-meshheading:17704754-CD8-Positive T-Lymphocytes,
pubmed-meshheading:17704754-Cancer Vaccines,
pubmed-meshheading:17704754-Dendritic Cells,
pubmed-meshheading:17704754-Gene Therapy,
pubmed-meshheading:17704754-Genetic Vectors,
pubmed-meshheading:17704754-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:17704754-Membrane Proteins,
pubmed-meshheading:17704754-Mice,
pubmed-meshheading:17704754-Mice, Inbred Strains,
pubmed-meshheading:17704754-Plasmids,
pubmed-meshheading:17704754-Receptor, erbB-2,
pubmed-meshheading:17704754-Spleen,
pubmed-meshheading:17704754-Tumor Burden,
pubmed-meshheading:17704754-Urinary Bladder Neoplasms,
pubmed-meshheading:17704754-Vaccination,
pubmed-meshheading:17704754-Vaccines, DNA
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| pubmed:year |
2007
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| pubmed:articleTitle |
Coexpression of Flt3 ligand and GM-CSF genes modulates immune responses induced by HER2/neu DNA vaccine.
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| pubmed:affiliation |
Institute of Basic Medical Sciences, National Cheng Kung University Medical College, Tainan, Taiwan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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