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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2007-9-17
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pubmed:abstractText |
Cyclic AMP (cAMP) receptor protein (CRP)/fumarate nitrate reductase regulator (FNR) family proteins are actively associated with defense against low oxygen stress, starvation and extreme temperature conditions. They are DNA-binding proteins and regulate target genes carrying the regulatory CRP/FNR cognate nucleotide sequence elements. Recombinant protein encoded by the Mycobacterium tuberculosis ORF Rv3676, a putative CRP/FNR regulator, was purified from Escherichia coli and was found to exist as dimer, devoid of any metal cation cofactor. Purified rRv3676 exhibited cAMP binding in a concentration-dependent manner. At lower concentrations of cAMP (6-10 microM) rRv3676 shows positive cooperativity; at 10 microM cAMP the protein exists in the most open conformation. rRv3676 could bind specifically to the putative CRP/FNR nucleotide sequence elements as evident from electrophoretic mobility shift assay.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1438-4221
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
297
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
451-7
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pubmed:meshHeading |
pubmed-meshheading:17702648-Bacterial Proteins,
pubmed-meshheading:17702648-Binding Sites,
pubmed-meshheading:17702648-Coenzymes,
pubmed-meshheading:17702648-Cyclic AMP,
pubmed-meshheading:17702648-DNA, Bacterial,
pubmed-meshheading:17702648-Dimerization,
pubmed-meshheading:17702648-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:17702648-Escherichia coli,
pubmed-meshheading:17702648-Metals,
pubmed-meshheading:17702648-Mycobacterium tuberculosis,
pubmed-meshheading:17702648-Protein Binding,
pubmed-meshheading:17702648-Protein Conformation,
pubmed-meshheading:17702648-Recombinant Proteins,
pubmed-meshheading:17702648-Transcription Factors
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pubmed:year |
2007
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pubmed:articleTitle |
Novel biochemical properties of a CRP/FNR family transcription factor from Mycobacterium tuberculosis.
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pubmed:affiliation |
Laboratory of Molecular and Cellular Biology, CDFD, Hyderabad 500076, India.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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