Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2007-10-19
pubmed:abstractText
The specific mechanisms underlying Taenia solium oncosphere adherence and penetration in the host have not been studied previously. We developed an in vitro adhesion model assay to evaluate the mechanisms of T. solium oncosphere adherence to the host cells. The following substrates were used: porcine intestinal mucosal scrapings (PIMS), porcine small intestinal mucosal explants (PSIME), Chinese hamster ovary cells (CHO cells), epithelial cells from ileocecal colorectal adenocarcinoma (HCT-8 cells), and epithelial cells from colorectal adenocarcinoma (Caco-2 cells). CHO cells were used to compare oncosphere adherence to fixed and viable cells, to determine the optimum time of oncosphere incubation, to determine the role of sera and monolayer cell maturation, and to determine the effect of temperature on oncosphere adherence. Light microscopy, scanning microscopy, and transmission microscopy were used to observe morphological characteristics of adhered oncospheres. This study showed in vitro adherence of activated T. solium oncospheres to PIMS, PSIME, monolayer CHO cells, Caco-2 cells, and HCT-8 cells. The reproducibility of T. solium oncosphere adherence was most easily measured with CHO cells. Adherence was enhanced by serum-binding medium with >5% fetal bovine serum, which resulted in a significantly greater number of oncospheres adhering than the number adhering when serum at a concentration less than 2.5% was used (P < 0.05). Oncosphere adherence decreased with incubation of cells at 4 degrees C compared with the adherence at 37 degrees C. Our studies also demonstrated that T. solium oncospheres attach to cells with elongated microvillus processes and that the oncospheres expel external secretory vesicles that have the same oncosphere processes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-10524964, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-10618076, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-12191899, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-13730457, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-14640505, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-15043964, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-15273394, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-15463066, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-1678809, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-2416306, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-2543634, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-2896759, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-2985503, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-3257206, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-3438292, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-3583538, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-5673256, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-5724486, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-6150008, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-6271810, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-6390196, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-6685462, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-6988694, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-7119990, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-7199506, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-7483769, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-7731918, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-7928063, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-8437052, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-8512570, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-852517, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-9095285, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-958404, http://linkedlifedata.com/resource/pubmed/commentcorrection/17698575-9715933
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5158-66
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Taenia solium oncosphere adhesion to intestinal epithelial and Chinese hamster ovary cells in vitro.
pubmed:affiliation
Department of Microbiology, Universidad Peruana Cayetano Heredia, P.O. Box 5045, Lima, Peru.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural