Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2007-8-16
pubmed:abstractText
Vascular remodeling is a common feature of many vasculopathies, including graft arteriosclerosis (GA). We investigated whether endothelial and smooth muscle cell-derived neuropilin-like protein (ESDN) is a marker of vascular remodeling in GA. Immunostaining of human coronary arteries demonstrated high levels of ESDN in GA, but not in normal arteries. In a model of GA, where a segment of human coronary is transplanted into a severe combined immunodeficient mouse, followed by allogeneic human peripheral blood mononuclear cell (PBMC) reconstitution, ESDN was minimally expressed in transplanted human arteries in the absence of reconstitution. By 2 weeks following PBMC reconstitution, at a time corresponding to maximal vascular cell proliferation, high levels of ESDN were detected in the transplanted arteries. Similarly, injury-induced vascular remodeling in apoE(-/-) mice was associated with early and transient ESDN upregulation, in parallel with cell proliferation. In vascular smooth muscle cell (VSMC) cultures, ESDN expression was significantly higher in proliferating, as compared to growth-arrested cells. ESDN overexpression in VSMC led to a decline in growth curves, while ESDN knock down had the opposite effect. We conclude that ESDN is a marker of vascular remodeling and regulator of VSMC proliferation. ESDN may serve as a therapeutic or diagnostic target for GA.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1600-6135
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2098-105
pubmed:dateRevised
2010-5-26
pubmed:meshHeading
pubmed-meshheading:17697260-Animals, pubmed-meshheading:17697260-Biological Markers, pubmed-meshheading:17697260-Cell Proliferation, pubmed-meshheading:17697260-Cells, Cultured, pubmed-meshheading:17697260-Coronary Artery Disease, pubmed-meshheading:17697260-Coronary Vessels, pubmed-meshheading:17697260-Disease Models, Animal, pubmed-meshheading:17697260-Humans, pubmed-meshheading:17697260-Immunohistochemistry, pubmed-meshheading:17697260-Membrane Proteins, pubmed-meshheading:17697260-Mice, pubmed-meshheading:17697260-Mice, SCID, pubmed-meshheading:17697260-Muscle, Smooth, Vascular, pubmed-meshheading:17697260-RNA, pubmed-meshheading:17697260-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:17697260-Tissue Transplantation, pubmed-meshheading:17697260-Transplantation, Homologous, pubmed-meshheading:17697260-Up-Regulation
pubmed:year
2007
pubmed:articleTitle
ESDN is a marker of vascular remodeling and regulator of cell proliferation in graft arteriosclerosis.
pubmed:affiliation
Raymond and Beverly Sackler Cardiovascular Molecular Imaging Laboratory, Yale University School of Medicine, New Haven, CT, USA. Mehran.sadeghi@yale.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural