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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1992-2-26
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pubmed:abstractText |
Injection of C5-sufficient BALB/c serum rendered DBA/2 mice (C5-deficient) immunologically hypo- or non-responsive to C5. This was indicated by C5-elimination studies in the C5-deficient mice showing similar half-lives for C5 upon single and repeated BALB/c serum injection. Concrete evidence for C5 non-responsiveness came from experiments showing that C5-injected DBA/2 mice were unable to mount an anti-C5 antibody response after active immunization with C5-sufficient serum in Freund's complete adjuvant. C5 hypo/non-responsiveness could be induced in DBA/2 mice via the intravenous as well as the intraperitoneal route, provided the C5-sufficient serum was administered in the very narrow dose range of 10-100 microliters (approximately 0.3-3 micrograms of C5). Upon i.v. C5 injection, C5 non-responsiveness was nearly complete on Day 4 and lasted about 3 weeks. Hyporesponsiveness was still present 6 weeks after serum injection. C3-/C5-depleting cobra venom factor reversed tolerization for C5, at least when applied within 48 hr after i.v. C5 injection. Similarity between the acquired C5 hypo/non-responsiveness of DBA/2 mice and the established C5 tolerance of BALB/c mice was suggested by adoptive cell transfer experiments: spleen cells from naive DBA/2 mice stimulated B cells of C5-sufficient nude mice to produce C5-neutralizing antibodies. In contrast, splenocytes from C5-tolerized DBA/2 mice, like those of BALB/c mice, did not decrease haemolytic C5 levels in C5-sufficient nude mice.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-14247728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-2031152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-2525422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-2586604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-2586609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-2653373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-2760478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-2784102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-2909657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-2945729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-2965023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-3260350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-3819435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-4125922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-5640947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-5766347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-6399977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-6619553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-6808076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1769686-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
380-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1769686-Animals,
pubmed-meshheading:1769686-Autoantibodies,
pubmed-meshheading:1769686-Complement C5,
pubmed-meshheading:1769686-Dose-Response Relationship, Immunologic,
pubmed-meshheading:1769686-Immune Tolerance,
pubmed-meshheading:1769686-Immunoglobulin G,
pubmed-meshheading:1769686-Mice,
pubmed-meshheading:1769686-Mice, Inbred BALB C,
pubmed-meshheading:1769686-Mice, Inbred DBA,
pubmed-meshheading:1769686-Time Factors
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pubmed:year |
1991
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pubmed:articleTitle |
Induction of active immunological hypo/non-responsiveness to C5 in adult C5-deficient DBA/2 mice.
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pubmed:affiliation |
Department of Experimental Microbiology, University Hospital, Utrecht, The Netherlands.
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pubmed:publicationType |
Journal Article
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