Linked Life Data

17693709

Source:http://linkedlifedata.com/resource/pubmed/id/17693709

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2007-8-13
pubmed:abstractText
The expression of iron-regulated genes in bacteria is typically controlled by the ferric uptake regulator (Fur) protein, a global transcriptional repressor that regulates functions as diverse as iron acquisition, oxidative stress, virulence and acid tolerance. We have identified a fur homologue in Vibrio alginolyticus and shown that it complements an Escherichia coli fur mutant. Reverse transcriptase PCR (RT-PCR) analysis proved that unlike many other fur homologues, V. alginolyticusfur is not under the iron-response Fur autoregulation. Homology modeling of the V. alginolyticus Fur protein with the recently solved crystal structure of Fur from Pseudomonas aeruginosa indicated extensive structural conservation. Based on the highly conserved DNA-binding sites and metal-binding sites in Fur protein, a series of site-directed mutations were respectively introduced into the cloned V. alginolyticus fur gene and resulted in partial or complete loss of Fur repressor function. Mutations in H33 and H90 were associated with complete loss of Fur function, mutations in Y56, R57, H87, C93 and H125 were related to partial loss of Fur function, and mutations in C96 and C133 did not show obvious change of Fur function. Our studies allowed the localization of some essential amino acid sites which may play important structural or functional roles in V. alginolyticus Fur activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1464-1801
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2007 S. Karger AG, Basel.
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15-21
pubmed:dateRevised
2010-10-19
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Characterization of the Vibrio alginolyticusfur gene and localization of essential amino acid sites in fur by site-directed mutagenesis.
pubmed:affiliation
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
pubmed:publicationType
Journal Article