|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2007-8-10
|
|
pubmed:abstractText |
Previous work in our laboratory demonstrated the existence of an association between heat shock transcription factor 2 (HSF2) and the serine/threonine phosphatase 2A, which is mediated by interaction between HSF2 and the A subunit (also called PR65) of this protein phosphatase. In light of the importance of HSF2-PP2A association for HSF2 cellular function, in this study, we have sought to dissect the sequences within HSF2 that are important for interaction with the A subunit of PP2A. The results of these experiments indicate that the HSF2 region comprising amino acids 343-363 is important for A subunit interaction. This region includes part of the C-terminal leucine zipper motif of HSF2 called heptad repeat C (HR-C). The results of transfection/immunoprecipitation experiments also show that deletion of the 6 amino acids from 343 to 348 from HSF2 (HSF2 (delta343-348)), is sufficient to prevent HSF2 from interacting with PP2A. These data provide insight into a new functional domain of HSF2, the PP2A A subunit-interacting region.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17688198-10224043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17688198-10872807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17688198-11278381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17688198-11344080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17688198-12388751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17688198-15497499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17688198-15662014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17688198-16188444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17688198-1717345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17688198-1871105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17688198-1871106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17688198-7553870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17688198-8441385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17688198-9278053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17688198-9619097
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
1355-8145
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
12
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
192-7
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:17688198-Amino Acid Sequence,
pubmed-meshheading:17688198-Binding Sites,
pubmed-meshheading:17688198-Green Fluorescent Proteins,
pubmed-meshheading:17688198-HeLa Cells,
pubmed-meshheading:17688198-Heat-Shock Proteins,
pubmed-meshheading:17688198-Humans,
pubmed-meshheading:17688198-Molecular Sequence Data,
pubmed-meshheading:17688198-Phosphoprotein Phosphatases,
pubmed-meshheading:17688198-Protein Binding,
pubmed-meshheading:17688198-Protein Phosphatase 2,
pubmed-meshheading:17688198-Structure-Activity Relationship,
pubmed-meshheading:17688198-Transcription Factors,
pubmed-meshheading:17688198-Two-Hybrid System Techniques
|
|
pubmed:year |
2007
|
|
pubmed:articleTitle |
Identification of the PP2A-interacting region of heat shock transcription factor 2.
|
|
pubmed:affiliation |
Department of Molecular and Cellular Biochemistry, University of Kentucky, 741 S Limestone Streeet, Lexington, KY 40536, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
|
