Linked Life Data

17685411

Source:http://linkedlifedata.com/resource/pubmed/id/17685411

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-12-20
pubmed:abstractText
A reliable and rapid method based on rapid-resolution liquid chromatography-diode array detection (RRLC-DAD) and electrospray ionization time-of-flight mass spectrometry (ESI-TOF/MS) has been developed for the isolation and characterization of multiple constituents in the root of Stellera chamaejasme L., which was extracted by sonication with methanol in an optimized procedure. Separation of the multiple constituents was achieved on an Agilent Zorbax XDB-C18 (50x3.0 mm i.d.; 1.8 microm) column using a gradient elution at a flow rate of 0.4 mL/min. The detection wavelength was 210 nm. Mass spectra were acquired in both positive and negative modes. A formula database of the known chemical constituents in the root of Stellera chamaejasme L. was established by an Agilent software. Twenty-two obvious peaks appeared in the total ion chromatogram and nine of them were characterized by TOF/MS. The RRLC-DAD and ESI-TOF/MS method with ultrasonic extraction would be useful for rapid and effective characterization of chemical constituents in the root of Stellera chamaejasme L.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0269-3879
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2007 John Wiley & Sons, Ltd.
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
64-72
pubmed:meshHeading
pubmed-meshheading:17685411-Chromatography, High Pressure Liquid, pubmed-meshheading:17685411-Coumarins, pubmed-meshheading:17685411-Diterpenes, pubmed-meshheading:17685411-Drugs, Chinese Herbal, pubmed-meshheading:17685411-Flavonoids, pubmed-meshheading:17685411-Glycosides, pubmed-meshheading:17685411-Lasers, Semiconductor, pubmed-meshheading:17685411-Lignans, pubmed-meshheading:17685411-Methanol, pubmed-meshheading:17685411-Molecular Weight, pubmed-meshheading:17685411-Plant Roots, pubmed-meshheading:17685411-Reproducibility of Results, pubmed-meshheading:17685411-Spectrometry, Mass, Electrospray Ionization, pubmed-meshheading:17685411-Spectrophotometry, Ultraviolet, pubmed-meshheading:17685411-Stereoisomerism, pubmed-meshheading:17685411-Tandem Mass Spectrometry, pubmed-meshheading:17685411-Thymelaeaceae
pubmed:year
2008
pubmed:articleTitle
Characterization of constituents in Stellera chamaejasme L. by rapid-resolution liquid chromatography-diode array detection and electrospray ionization time-of-flight mass spectrometry.
pubmed:affiliation
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, and Department of Pharmacy, Eastern Heptatobiliary Surgery Hospital, Shanghai 200433, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't