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rdf:type |
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lifeskim:mentions |
umls-concept:C0003316,
umls-concept:C0019682,
umls-concept:C0031928,
umls-concept:C0151332,
umls-concept:C0205147,
umls-concept:C0237401,
umls-concept:C0750491,
umls-concept:C0871261,
umls-concept:C1418426,
umls-concept:C1704632,
umls-concept:C1705241,
umls-concept:C1705242,
umls-concept:C1706817,
umls-concept:C2911692
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pubmed:issue |
1
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pubmed:dateCreated |
2007-9-12
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pubmed:abstractText |
Tuberculosis is the most frequent co-infection in human immunodeficiency virus (HIV)-infected individuals, and which still presents diagnostic difficulties. Recently we set up an assay based on interferon (IFN)-gamma response to region of difference 1 (RD1) peptides selected by computational analysis which is associated with active Mycobacterium tuberculosis replication. The objective of this study was to investigate the response to RD1 selected peptides in HIV-1-infected individuals in a clinical setting. The mechanisms of this immune response and comparison with other immune assays were also investigated. A total of 111 HIV-infected individuals with symptoms and signs consistent with active tuberculosis were enrolled prospectively. Interferon (IFN)-gamma responses to RD1 selected peptides and recall antigens were evaluated by enzyme-linked immunospot assay. Results were correlated with CD4(+) T cell counts, individuals' characteristics, tuberculin skin test, QuantiFERON-TB Gold and T-SPOT.TB. Results from 21 (19%) individuals were indeterminate due to in vitro cell anergy. Among 'non-anergic' individuals, sensitivity for active tuberculosis of the assay based on RD1 selected peptides was 67% (24 of 36), specificity was 94% (three of 54). The assay also resulted positive in cases of extra-pulmonary and smear-negative pulmonary active tuberculosis. The response was mediated by CD4(+) effector/memory T cells and correlated with CD4(+) T cell counts, but not with plasma HIV-RNA load. Moreover, the RD1 selected peptides assay had the highest diagnostic odds ratio for active tuberculosis compared to tuberculin skin test (TST), QuantiFERON-TB Gold and T-SPOT.TB. RD1 selected peptides assay is associated with M. tuberculosis replication in HIV-infected individuals, although T cell anergy remains an important obstacle to be overcome before the test can be proposed as a diagnostic tool.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-10348738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-10517722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-10587318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-10903781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-11064664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-11133379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-11826002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-12165552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-12441800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-12461417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-12865946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-14986262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-15059788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-15293599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-15567126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-15610806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-16002712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-16200083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-16260914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-16275946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-16288403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-16579856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-16700703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-16960787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-17143823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-17158278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-17218620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-17255757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-17257436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-7621756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-7729876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-8110954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-9154896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-9207360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-9846755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-9862331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17680823-9952370
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
150
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
91-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17680823-Adult,
pubmed-meshheading:17680823-Antigens, Bacterial,
pubmed-meshheading:17680823-Bacterial Proteins,
pubmed-meshheading:17680823-CD4 Lymphocyte Count,
pubmed-meshheading:17680823-CD4-Positive T-Lymphocytes,
pubmed-meshheading:17680823-Clonal Anergy,
pubmed-meshheading:17680823-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:17680823-Epitopes,
pubmed-meshheading:17680823-Female,
pubmed-meshheading:17680823-HIV Infections,
pubmed-meshheading:17680823-HIV-1,
pubmed-meshheading:17680823-Humans,
pubmed-meshheading:17680823-Interferon-gamma,
pubmed-meshheading:17680823-Male,
pubmed-meshheading:17680823-Mycobacterium tuberculosis,
pubmed-meshheading:17680823-Pilot Projects,
pubmed-meshheading:17680823-Prospective Studies,
pubmed-meshheading:17680823-Tuberculin Test,
pubmed-meshheading:17680823-Tuberculosis
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pubmed:year |
2007
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pubmed:articleTitle |
Response to region of difference 1 (RD1) epitopes in human immunodeficiency virus (HIV)-infected individuals enrolled with suspected active tuberculosis: a pilot study.
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pubmed:affiliation |
Translational Research Unit, Department of Experimental Research, Istituto Nazionale Malattie Infettive Lazzaro Spallanzani- IRCCS Rome, Italy.
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