Linked Life Data

17674780

Source:http://linkedlifedata.com/resource/pubmed/id/17674780

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-8-6
pubmed:abstractText
AtMAP65-1 gene was cloned from Arabidopsis genome. A plant expression vector containing AtMAP65-1-GFP fusion gene driven by 35S promoter was constructed and introduced into Arabidopsis by Agrobacterium infection. Under confocal laser scanning microscope (CLSM), it was found that in the light-induced opening guard cells AtMAP65-1 was distributed radiately from dorsal wall to ventral wall in a bundling way. Treated with microtubule specific inhibitor-Vinblastine (10 micromol/L) for 90 min, the organization of AtMAP65-1 in guard cell was disrupted dramatically. Following the depolymerization of cortical microtubule and the decreasing of stomatal aperture, the fluorescence of AtMAP65-1 became to be fragmented into dotted spots. The organization of AtMAP65-1 was broken up completely when the treatment was up to 120 min. In the dark-induced closing guard cells, AtMAP65-1 was turned into fragments and was randomly dispersed throughout the cells. That is, AtMAP65-1 is co-localized with cortical microtubules. The results suggest that AtMAP65-1 binds cortical microtubules, and it may regulate the arrangement of cortical microtubules, which helps us gain more insights into the role of AtMAP65-1 in stomatal movement.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1673-520X
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
258-62
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
[Localization of microtubule-associated protein AtMAP65-1 in guard cells of arabidopsis].
pubmed:affiliation
College of Life Sciences,Capital Normal University, Beijing.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't