Linked Life Data

17674141

Source:http://linkedlifedata.com/resource/pubmed/id/17674141

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2008-12-5
pubmed:abstractText
OTUBAINS are a recently discovered family of cysteine proteases that participate in the ubiquitin pathway. These proteins were originally described in animal systems and are involved in removing the ubiquitin chain attached to a protein destined for degradation. In a cDNA-AFLP screen designed to identify genes that are expressed during early somatic embryogenesis in the conifer Pinus radiata, a fragment-derived transcript corresponding to an Otubain-like cysteine protease was identified. The full-length cDNA contained an 885 bp ORF encoding 294 amino acids, and was named PrOTUBAIN. The deduced protein showed high identity to other OTUBAINS and contained an OTU domain and a catalytic triad characteristic of cysteine proteases. The 3-D model of PrOTUBAIN showed significant similarity to human OTUBAIN2, suggesting that the plant protein may possess functions similar to that of the human protein. Real time PCR assays demonstrated that PrOTUBAIN is expressed in different tissues and that transcript are particularly abundant in embryogenic tissues. This is the first report of this class of protein in higher plants and the putative role of PrOTUBAIN is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0301-4851
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
567-73
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
A novel Otubain-like cysteine protease gene is preferentially expressed during somatic embryogenesis in Pinus radiata.
pubmed:affiliation
Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda 340, P.O. Box 114-D, Santiago, Chile.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't