Linked Life Data

17662454

Source:http://linkedlifedata.com/resource/pubmed/id/17662454

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-9-7
pubmed:abstractText
Mucosal tolerance induction with vaccines based on peptides representing T-cell epitopes of allergens is a promising way for treating allergic diseases. Ole e 1 is the main allergen of olive pollen, which is an important cause of allergy in Mediterranean countries. The aim of this study was to evaluate the ability of the peptide T109-K130 containing a dominant T-cell epitope of Ole e 1, to modulate the allergen-specific immune response in a prophylactic mouse model. Mice were intranasally treated with the peptide 1 week prior to sensitization with Ole e 1. Blood, lungs and spleens were collected and analysed for immune response. Intranasal pretreatment of mice with the peptide led to suppress serum specific IgE, IgG1 and IgG2a antibody levels, and markedly reduced proliferative T-cell response and Th2-cytokine production, but increased IFN-gamma secretion in spleen cell cultures. Increased mRNA IL-10 levels were observed in lungs from pretreated mice. Pathologic alterations of the lung associated with airway inflammation (peribronchial/perivascular infiltrates, eosinophilia and mucus production) were significantly suppressed after pretreatment. Similar results were obtained when mice were sensitized 10 weeks after treatment. Our results demonstrate that intranasal administration of a single T-cell peptide protects mice against subsequent sensitization to the allergen, possibly via IFN-gamma and IL-10. This study emphasizes the usefulness of nasal peptide T-based vaccines against allergy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0161-5890
pubmed:author
pubmed:issnType
Print
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
438-45
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:17662454-Administration, Intranasal, pubmed-meshheading:17662454-Allergens, pubmed-meshheading:17662454-Animals, pubmed-meshheading:17662454-Cell Proliferation, pubmed-meshheading:17662454-Epitopes, T-Lymphocyte, pubmed-meshheading:17662454-Female, pubmed-meshheading:17662454-Gene Expression Regulation, pubmed-meshheading:17662454-Humans, pubmed-meshheading:17662454-Hypersensitivity, pubmed-meshheading:17662454-Immune Tolerance, pubmed-meshheading:17662454-Immunization, pubmed-meshheading:17662454-Immunoglobulin E, pubmed-meshheading:17662454-Inflammation, pubmed-meshheading:17662454-Interferon-gamma, pubmed-meshheading:17662454-Interleukin-10, pubmed-meshheading:17662454-Lung, pubmed-meshheading:17662454-Mice, pubmed-meshheading:17662454-Mice, Inbred BALB C, pubmed-meshheading:17662454-Peptides, pubmed-meshheading:17662454-Plant Proteins, pubmed-meshheading:17662454-Pollen, pubmed-meshheading:17662454-Respiratory System, pubmed-meshheading:17662454-Time Factors, pubmed-meshheading:17662454-Transforming Growth Factor beta
pubmed:year
2008
pubmed:articleTitle
Intranasal immunization with a dominant T-cell epitope peptide of a major allergen of olive pollen prevents mice from sensitization to the whole allergen.
pubmed:affiliation
Departamento de Bioquímica y Biología Molecular, Facultad de Química, Universidad Complutense, 28040 Madrid, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't