Source:http://linkedlifedata.com/resource/pubmed/id/17662073
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
2007-12-18
|
pubmed:abstractText |
Listeria monocytogenes induces apoptosis in vitro and in vivo in a variety of cell types. However, the mechanism of cell death in L. monocytogenes-infected macrophages was initially reported to be distinct from apoptosis. Here, we studied the mechanism of L. monocytogenes-induced cell death using sensitive fluorescent techniques. We found that caspase-1 activation preceded cell death of macrophages infected with L. monocytogenes, using fluorogenic substrates. Caspase-1 activation was diminished after infection with wild-type L. monocytogenes when cells were treated with NH(4)Cl, or if they were infected with a listeriolysin mutant that cannot escape from the phagolysosome. Mitochondrial membrane integrity was preserved during the infection. A particular mechanism of cell death, recently termed 'pyroptosis', is associated with infection by intracellular microorganisms, and has an inherent pro-inflammatory character, due to involvement of caspase-1 activation with consequent IL-1 beta and IL-18 production. Cell death through caspase-1 activation would constitute a defence mechanism of macrophages which induces cell death to eliminate the bacteria's intracytosolic niche and recruits early host's defences through the secretion of inflammatory cytokines.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
1462-5822
|
pubmed:author | |
pubmed:issnType |
Electronic
|
pubmed:volume |
10
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
41-52
|
pubmed:meshHeading |
pubmed-meshheading:17662073-Animals,
pubmed-meshheading:17662073-Caspase 1,
pubmed-meshheading:17662073-Cell Death,
pubmed-meshheading:17662073-Cell Line,
pubmed-meshheading:17662073-Listeria monocytogenes,
pubmed-meshheading:17662073-Macrophages,
pubmed-meshheading:17662073-Mice,
pubmed-meshheading:17662073-Mitochondrial Membranes
|
pubmed:year |
2008
|
pubmed:articleTitle |
Intracytosolic Listeria monocytogenes induces cell death through caspase-1 activation in murine macrophages.
|
pubmed:affiliation |
Department of Infectious Diseases, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|