Linked Life Data

17661469

Source:http://linkedlifedata.com/resource/pubmed/id/17661469

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
33
pubmed:dateCreated
2007-8-16
pubmed:abstractText
The design of variable-stability coiled-coil heterodimers is described. The electrostatic interface between helices, formed by contact between side chains in heptad e/g positions, is manipulated to produce complexes ranging in stability from ones that are essentially unstructured to those that cannot be thermally denatured. The tuning is accomplished by incremental extension or contraction of parent glutamic acid and lysine side chains by single methylene units, producing peptides that bear either carboxylic acids or amines separated from the peptide backbone by one to four CH2 groups. Detailed examination of all homodimers and electrostatically compatible heterodimers generates interesting combinations, particularly those in which longer-chain acids are incorporated into peptides paired with lysine-bearing ones. The discovery of very stable dimers allows exchange experiments in which one strand of an original heterodimer is specifically replaced by an added one, even in cases where the original complex features the native-like glutamic acid/lysine pairing. The reported results add significantly to the available design templates for coiled-coil construction and enable the future implementation of various triggered-recognition strategies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0002-7863
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
129
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10255-60
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Variable stability heterodimeric coiled-coils from manipulation of electrostatic interface residue chain length.
pubmed:affiliation
Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.