Source:http://linkedlifedata.com/resource/pubmed/id/17658619
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3-4
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| pubmed:dateCreated |
2007-10-30
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| pubmed:abstractText |
To study pathway specific gene expression within the immune-endocrine axis of dairy cows with Staphylococcus aureus mastitis, mRNA was collected from blood mononuclear cells (BMCs) and milk somatic cells (MSCs) of cows (n=7) identified as culture positive for S. aureus and their matched negative control cows (n=7) with no evidence of S. aureus mastitis. Labeled cDNA probes derived from BMCs and MSCs of infected and healthy cows were applied to a bovine immune-endocrine cDNA array containing 167 genes. Genes with a log(2) ratio> or =0.5 were considered to be up-regulated and genes with a log(2) ratio< or =-0.5 to be down-regulated. In total, 22 genes were differentially displayed in BMCs and 16 genes in MSCs of case versus controls. Expression of selected genes in BMCs and MSCs were confirmed by real-time PCR. The RT-PCR results were highly correlated with microarray measurements. Some of these genes, such as interleukin (IL)-8 have been previously implicated in other bacterial diseases, and are known to regulate immune responses; whereas, others may reflect novel pathways or genes involved in progressive mammary gland disease. For example, IL-18 was up-regulated in BMCs but not MSCs of mastitic quarters, while IL-17 was more highly expressed in MSCs compared to BMCs. This study identified a number of differentially expressed genes associated with bovine S. aureus mastitis and demonstrates the intricacy of the patterns of gene expression that influence host response to a complex pathogen of significant relevance to both human and veterinary medicine.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0165-2427
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
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| pubmed:volume |
120
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
201-11
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| pubmed:meshHeading |
pubmed-meshheading:17658619-Animals,
pubmed-meshheading:17658619-Antibodies, Bacterial,
pubmed-meshheading:17658619-Canada,
pubmed-meshheading:17658619-Case-Control Studies,
pubmed-meshheading:17658619-Cattle,
pubmed-meshheading:17658619-Cell Count,
pubmed-meshheading:17658619-Female,
pubmed-meshheading:17658619-Gene Expression Profiling,
pubmed-meshheading:17658619-Gene Expression Regulation,
pubmed-meshheading:17658619-Interleukin-18,
pubmed-meshheading:17658619-Mastitis, Bovine,
pubmed-meshheading:17658619-Milk,
pubmed-meshheading:17658619-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:17658619-Reproducibility of Results,
pubmed-meshheading:17658619-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17658619-Staphylococcal Infections,
pubmed-meshheading:17658619-Staphylococcus aureus
|
| pubmed:year |
2007
|
| pubmed:articleTitle |
Differentially expressed genes associated with Staphylococcus aureus mastitis of Canadian Holstein cows.
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| pubmed:affiliation |
Department of Pathobiology, University of Guelph, Guelph, Ontario, Canada N1G 2W1.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|