Source:http://linkedlifedata.com/resource/pubmed/id/17657222
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
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| pubmed:dateCreated |
2007-8-22
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| pubmed:abstractText |
The 8;21 translocation is a common chromosomal abnormality in acute myeloid leukemia (AML). We recently identified a naturally occurring leukemogenic splice variant, AML1-ETO9a (acute myeloid leukemia-1 transcription factor and the eight-twenty-one corepressor-9a), of t(8;21). To understand the leukemic potential of AML1-ETO9a, we performed microarray analysis with the murine multipotential hematopoietic FDCP-mix A4 cell line. We identified changes in expression of various genes including CD44. CD44 is a type I transmembrane protein and functions as the major cellular adhesion molecule for hyaluronic acid, a component of the extracellular matrix. CD44 is expressed in most human cell types and is implicated in myeloid leukemia pathogenesis. We show that the presence of AML1-ETO9a significantly increased the expression of CD44 at both RNA and protein levels. Furthermore, the CD44 promoter is bound by AML1-ETO9a and AML1-ETO at the chromatin level. In addition, in the AML1-ETO9a leukemia mouse model CD44 is regulated in a cell context-dependent manner. Thus, our observations suggest that AML1-ETO and its splice variant AML1-ETO9a are able to regulate the expression of the CD44 gene, linking the 8;21 translocation to the regulation of a cell adhesion molecule that is involved in the growth and maintenance of the AML blast/stem cells.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AML1-ETO fusion protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD44,
http://linkedlifedata.com/resource/pubmed/chemical/Core Binding Factor Alpha 2 Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins, Fusion
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0887-6924
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
21
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2010-9
|
| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:17657222-Acute Disease,
pubmed-meshheading:17657222-Alternative Splicing,
pubmed-meshheading:17657222-Animals,
pubmed-meshheading:17657222-Antigens, CD44,
pubmed-meshheading:17657222-Cell Differentiation,
pubmed-meshheading:17657222-Cell Division,
pubmed-meshheading:17657222-Cell Survival,
pubmed-meshheading:17657222-Chromosomes, Human, Pair 21,
pubmed-meshheading:17657222-Chromosomes, Human, Pair 8,
pubmed-meshheading:17657222-Core Binding Factor Alpha 2 Subunit,
pubmed-meshheading:17657222-Gene Expression Regulation, Leukemic,
pubmed-meshheading:17657222-Humans,
pubmed-meshheading:17657222-K562 Cells,
pubmed-meshheading:17657222-Leukemia, Myeloid,
pubmed-meshheading:17657222-Mice,
pubmed-meshheading:17657222-Neoplastic Stem Cells,
pubmed-meshheading:17657222-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:17657222-Oncogene Proteins, Fusion,
pubmed-meshheading:17657222-Translocation, Genetic
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| pubmed:year |
2007
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| pubmed:articleTitle |
The multi-functional cellular adhesion molecule CD44 is regulated by the 8;21 chromosomal translocation.
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| pubmed:affiliation |
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
|