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rdf:type |
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lifeskim:mentions |
umls-concept:C0007600,
umls-concept:C0017262,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0040649,
umls-concept:C0061101,
umls-concept:C0165519,
umls-concept:C0185117,
umls-concept:C0441889,
umls-concept:C0851285,
umls-concept:C2911684
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pubmed:issue |
4
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|
pubmed:dateCreated |
2007-7-26
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|
pubmed:abstractText |
Mouse FBJ virus-induced osteosarcoma FBJ-S1 cells rich in GD1a are not readily metastatic, whereas FBJ-LL cells with low levels of GD1a are highly metastatic. GD1a was previously shown to suppress metastasis of mouse FBJ cells and to upregulate caveolin-1 and stromal interaction molecule 1 expression. The present study demonstrates that matrix metalloproteinase-9 (MMP-9) expression renders FBJ-LL cells invasive. MMP-9 is inversely regulated by GD1a, based upon four observations: MMP-9 mRNA content was 5 times higher in FBJ-LL cells than FBJ-S1 cells; a GD1a-re-expressing FBJ-LL cell variant produced through beta1,4GalNAcT-1 cDNA transfection expressed lower levels of MMP-9; exogenous addition of GD1a to FBJ-LL cells decreased MMP-9 production in a dose- and time-dependent manner; and treatment of GD1a-rich cells with D-PDMP or siRNA targeting St3gal2 decreased GD1a expression, but augmented MMP-9 expression. This is the first report demonstrating that GD1a negatively regulates expression of MMP-9 at the transcriptional level.
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
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pubmed:status |
MEDLINE
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|
pubmed:issn |
0300-8207
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|
pubmed:author |
pubmed-author:BRUYY,
pubmed-author:HyugaSumikoS,
pubmed-author:ManZhiqiuZ,
pubmed-author:SatoToshinoriT,
pubmed-author:TakakuShizukaS,
pubmed-author:TanXuanX,
pubmed-author:WangPuP,
pubmed-author:WangXiaodongX,
pubmed-author:XinshengYaoY,
pubmed-author:YamagataTatsuyaT,
pubmed-author:YooChul-BaeCB
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|
pubmed:issnType |
Print
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pubmed:volume |
48
|
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pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
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pubmed:pagination |
198-205
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pubmed:meshHeading |
pubmed-meshheading:17653976-Animals,
pubmed-meshheading:17653976-Cell Line, Tumor,
pubmed-meshheading:17653976-Cell Movement,
pubmed-meshheading:17653976-Dose-Response Relationship, Drug,
pubmed-meshheading:17653976-Gangliosides,
pubmed-meshheading:17653976-Gene Expression Regulation,
pubmed-meshheading:17653976-Matrix Metalloproteinase 9,
pubmed-meshheading:17653976-Mice,
pubmed-meshheading:17653976-Morpholines,
pubmed-meshheading:17653976-Neoplasm Invasiveness,
pubmed-meshheading:17653976-Neoplasm Metastasis,
pubmed-meshheading:17653976-Osteosarcoma,
pubmed-meshheading:17653976-RNA, Messenger,
pubmed-meshheading:17653976-RNA, Small Interfering,
pubmed-meshheading:17653976-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17653976-Transcription, Genetic
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pubmed:year |
2007
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|
pubmed:articleTitle |
Ganglioside GD1a negatively regulates matrix metalloproteinase-9 expression in mouse FBJ cell lines at the transcriptional level.
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pubmed:affiliation |
Laboratory of Tumor Biology and Glycobiology, Shenyang Pharmaceutical University, Shenyang, P. R. China.
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pubmed:publicationType |
Journal Article
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