Source:http://linkedlifedata.com/resource/pubmed/id/17653084
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
|
pubmed:dateCreated |
2008-1-31
|
pubmed:abstractText |
The availability of the eukaryotic polypeptide chain initiation factor 4E (eIF4E) for protein synthesis is regulated by the 4E-binding proteins (4E-BPs), which act as inhibitors of cap-dependent mRNA translation. The ability of the 4E-BPs to sequester eIF4E is regulated by reversible phosphorylation at multiple sites. We show here that, in addition, 4E-BP1 is a substrate for polyubiquitination and that some forms of 4E-BP1 are simultaneously polyubiquitinated and phosphorylated. In Jurkat cells inhibition of proteasomal activity by MG132 enhances the level of hypophosphorylated, unmodified 4E-BP1 but only modestly increases the accumulation of high-molecular-weight, phosphorylated forms of 4E-BP1. In contrast, inhibition of protein phosphatase activity with calyculin A reduces the level of unmodified 4E-BP1 but strongly enhances the amount of phosphorylated, high-molecular-weight 4E-BP1. Turnover measurements in the presence of cycloheximide show that, whereas 4E-BP1 is normally a very stable protein, calyculin A decreases the apparent half-life of the normal-sized protein. Affinity chromatography on m(7)GTP-Sepharose indicates that the larger forms of 4E-BP1 bind very poorly to eIF4E. We suggest that the phosphorylation of 4E-BP1 may play a dual role in the regulation of protein synthesis, both reducing the affinity of 4E-BP1 for eIF4E and promoting the conversion of 4E-BP1 to alternative, polyubiquitinated forms.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Proteinase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/EIF4EBP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Eukaryotic Initiation Factor-4E,
http://linkedlifedata.com/resource/pubmed/chemical/Leupeptins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteasome Endopeptidase Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin,
http://linkedlifedata.com/resource/pubmed/chemical/benzyloxycarbonylleucyl-leucyl-leuci...
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
1476-5594
|
pubmed:author | |
pubmed:issnType |
Electronic
|
pubmed:day |
31
|
pubmed:volume |
27
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
811-22
|
pubmed:meshHeading |
pubmed-meshheading:17653084-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:17653084-Animals,
pubmed-meshheading:17653084-Cysteine Proteinase Inhibitors,
pubmed-meshheading:17653084-Eukaryotic Initiation Factor-4E,
pubmed-meshheading:17653084-Humans,
pubmed-meshheading:17653084-Jurkat Cells,
pubmed-meshheading:17653084-Leupeptins,
pubmed-meshheading:17653084-Mice,
pubmed-meshheading:17653084-Molecular Weight,
pubmed-meshheading:17653084-Phosphoproteins,
pubmed-meshheading:17653084-Phosphorylation,
pubmed-meshheading:17653084-Proteasome Endopeptidase Complex,
pubmed-meshheading:17653084-Protein Biosynthesis,
pubmed-meshheading:17653084-Ubiquitin
|
pubmed:year |
2008
|
pubmed:articleTitle |
Effects of protein phosphorylation on ubiquitination and stability of the translational inhibitor protein 4E-BP1.
|
pubmed:affiliation |
Translational Control Group, Division of Basic Medical Sciences, Centre for Molecular and Metabolic Signalling, St George's, University of London, London, UK.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|