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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0043343,
umls-concept:C0059036,
umls-concept:C0204727,
umls-concept:C0205409,
umls-concept:C0300140,
umls-concept:C0679058,
umls-concept:C1171362,
umls-concept:C1515670,
umls-concept:C1527148,
umls-concept:C1547699,
umls-concept:C1880022,
umls-concept:C2003941,
umls-concept:C2700640
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|
pubmed:issue |
2
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|
pubmed:dateCreated |
1992-2-18
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pubmed:databankReference |
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|
pubmed:abstractText |
In Xenopus laevis, the gene encoding the elongation factor 1-alpha variant EF-1 alpha O, where O stands for oocyte, is expressed in oocytes and early embryos. A genomic library from X. laevis was screened with a cDNA probe coding for EF-1 alpha O. Two recombinant phages were isolated, one of which carries an entire EF-1 alpha O gene. This clone was characterized by restriction enzyme mapping and sequencing. Comparison of cDNA and genomic sequences revealed that EF-1 alpha O consists of seven exons spanning about 6.5 kb. The structure of the gene is very homologous to the human EF-1 alpha gene, as all locations of the splice junctions are conserved between the two genes. The sequence immediately upstream from the transcription start point (tsp) contains a CCAAT box, but does not contain either a TATA box or a Sp1-binding site. Interestingly, this sequence has a sequence homologous to the negative regulatory element from the TFIIIA promoter. A region located about 400 bp upstream from the tsp contains an additional number of possible regulatory sequence elements. The first intron contains G + C-rich elements which exist both isolated and as part of longer inverted repeats. Furthermore, one octamer and four Sp1-binding sites are found in this intron.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0378-1119
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
109
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pubmed:geneSymbol |
EF-1&agr;O
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
185-92
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1765266-Amino Acid Sequence,
pubmed-meshheading:1765266-Animals,
pubmed-meshheading:1765266-Base Sequence,
pubmed-meshheading:1765266-Cloning, Molecular,
pubmed-meshheading:1765266-Enhancer Elements, Genetic,
pubmed-meshheading:1765266-Exons,
pubmed-meshheading:1765266-Gene Expression,
pubmed-meshheading:1765266-Introns,
pubmed-meshheading:1765266-Molecular Sequence Data,
pubmed-meshheading:1765266-Oocytes,
pubmed-meshheading:1765266-Peptide Elongation Factor 1,
pubmed-meshheading:1765266-Peptide Elongation Factors,
pubmed-meshheading:1765266-Promoter Regions, Genetic,
pubmed-meshheading:1765266-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:1765266-Restriction Mapping,
pubmed-meshheading:1765266-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1765266-Transcription, Genetic,
pubmed-meshheading:1765266-Transcription Factor TFIIIA,
pubmed-meshheading:1765266-Transcription Factors,
pubmed-meshheading:1765266-Xenopus laevis
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pubmed:year |
1991
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pubmed:articleTitle |
Isolation and characterization of the gene encoding EF-1 alpha O, an elongation factor 1-alpha expressed during early development of Xenopus laevis.
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pubmed:affiliation |
Division of Biostructural Chemistry, Aarhus University, Denmark.
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pubmed:publicationType |
Journal Article
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