Source:http://linkedlifedata.com/resource/pubmed/id/17645941
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
30
|
| pubmed:dateCreated |
2007-8-13
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| pubmed:abstractText |
The inflammatory response is a key component in the biocompatibility of biomaterials. Among the factors that control the development of inflammation is a critical molecule nuclear factor-kappaB (NF-kappaB). Therefore, the aim of this study was to assess the feasibility of noninvasive whole-body real-time imaging for the evaluation of host-biomaterial interaction in the NF-kappaB transgenic mice. Transgenic mice, carrying the luciferase gene under the control of NF-kappaB, were constructed. In vivo bioluminescence imaging showed that the constitutive and induced NF-kappaB activities of transgenic mice were detected in most of the lymphoid tissues, demonstrating that NF-kappaB-driven luminescence reflected the inflammatory response in vivo. By the implantation of genipin-cross-linked gelatin conduit (GGC) and bacterial endotoxin-immersed GGC in the dorsal region, we detected a strong and specific luminescent signal from the tissue around the bacterial endotoxin-immersed GGC implant. Histological and immunohistochemical analysis also demonstrated that inflammation, characterized by the infiltration of immune cells, the accumulation of fluid, and the activation of NF-kappaB, was evoked around the same region. The correlation between the bioluminescence imaging and histological changes indicated that noninvasive imaging technique could be used to monitor the real-time inflammation in the implanted mice.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biocompatible Materials,
http://linkedlifedata.com/resource/pubmed/chemical/Gelatin,
http://linkedlifedata.com/resource/pubmed/chemical/Iridoid Glycosides,
http://linkedlifedata.com/resource/pubmed/chemical/Iridoids,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/genipin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
|
| pubmed:issn |
0142-9612
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
28
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4370-7
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:17645941-Animals,
pubmed-meshheading:17645941-Biocompatible Materials,
pubmed-meshheading:17645941-Diagnostic Imaging,
pubmed-meshheading:17645941-Feasibility Studies,
pubmed-meshheading:17645941-Gelatin,
pubmed-meshheading:17645941-Gene Expression Regulation,
pubmed-meshheading:17645941-Genes, Reporter,
pubmed-meshheading:17645941-Immunohistochemistry,
pubmed-meshheading:17645941-Inflammation,
pubmed-meshheading:17645941-Iridoid Glycosides,
pubmed-meshheading:17645941-Iridoids,
pubmed-meshheading:17645941-Luciferases,
pubmed-meshheading:17645941-Luminescent Measurements,
pubmed-meshheading:17645941-Materials Testing,
pubmed-meshheading:17645941-Mice,
pubmed-meshheading:17645941-Mice, Transgenic,
pubmed-meshheading:17645941-Microinjections,
pubmed-meshheading:17645941-NF-kappa B,
pubmed-meshheading:17645941-Prostheses and Implants
|
| pubmed:year |
2007
|
| pubmed:articleTitle |
Noninvasive nuclear factor-kappaB bioluminescence imaging for the assessment of host-biomaterial interaction in transgenic mice.
|
| pubmed:affiliation |
Molecular Biology Laboratory, Graduate Institute of Chinese Medical Science, China Medical University, Taichung 40402, Taiwan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|