Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2007-9-12
pubmed:abstractText
The fosABCDXE operon encodes components of a putative fructose/mannose phosphoenolpyruvate-dependent phosphotransferase system and a beta-fructosidase precursor (FosE) that are involved in the fructooligosaccharide (FOS) utilization pathway of Lactobacillus paracasei 1195. The presence of an N-terminal signal peptide sequence and an LPQAG cell wall anchor motif in the C-terminal region of the deduced FosE precursor amino acid sequence predicted that the enzyme is cell wall associated, indicating that FOS may be hydrolyzed extracellularly. In this study, cell fractionation experiments demonstrated that the FOS hydrolysis activity was present exclusively in the cell wall extract of L. paracasei previously grown on FOS. In contrast, no measurable FOS hydrolysis activity was detected in the cell wall extract from the isogenic fosE mutant. Induction of beta-fructosidase activity was observed when cells were grown on FOS, inulin, sucrose, or fructose but not when cells were grown on glucose. A diauxic growth pattern was observed when cells were grown on FOS in the presence of limiting glucose (0.1%). Analysis of the culture supernatant revealed that glucose was consumed first, followed by the longer-chain FOS species. Transcription analysis further showed that the fos operon was expressed only after glucose was depleted in the medium. Expression of fosE in a non-FOS-fermenting strain, Lactobacillus rhamnosus GG, enabled the recombinant strain to metabolize FOS, inulin, sucrose, and levan.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-10217795, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-10532392, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-10618232, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-10721914, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-10831458, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-10896218, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-12086179, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-12095678, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-12381787, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-12460425, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-12676703, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-12732943, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-12754227, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-12847288, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-15214632, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-15231787, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-1537524, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-15995208, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-16269678, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-17028235, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-17261521, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-1728516, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-1900939, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-1924373, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-2117666, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-2495266, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-7144579, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-7592486, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-7698613, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-7782892, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-7830549, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-7920415, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-8013867, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-8299952, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-8615353, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-9600614, http://linkedlifedata.com/resource/pubmed/commentcorrection/17644636-9622354
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
73
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5716-24
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Functional analysis of the fructooligosaccharide utilization operon in Lactobacillus paracasei 1195.
pubmed:affiliation
University of Nebraska, Department of Food Science and Technology, 338 FIC, Lincoln, NE 68583-0919, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't